Advanced cell culture techniques for cancer drug discovery

CJ Lovitt, TB Shelper, VM Avery - Biology, 2014 - mdpi.com
Human cancer cell lines are an integral part of drug discovery practices. However, modeling
the complexity of cancer utilizing these cell lines on standard plastic substrata, does not …

qPCR-based methods for expression analysis of miRNAs

DA Forero, Y González-Giraldo, LJ Castro-Vega… - …, 2019 - Taylor & Francis
Several approaches for miRNA expression analysis have been developed in recent years.
In this article, we provide an updated and comprehensive review of available qPCR-based …

[HTML][HTML] Caudovirales bacteriophages are associated with improved executive function and memory in flies, mice, and humans

J Mayneris-Perxachs, A Castells-Nobau… - Cell Host & Microbe, 2022 - cell.com
Growing evidence implicates the gut microbiome in cognition. Viruses, the most abundant
life entities on the planet, are a commonly overlooked component of the gut virome …

MicroRNAs in Alzheimer's disease: differential expression in hippocampus and cell-free cerebrospinal fluid

M Müller, HB Kuiperij, JA Claassen, B Küsters… - Neurobiology of …, 2014 - Elsevier
MicroRNAs (miRNAs) are small, noncoding RNAs that function in complex networks to
regulate protein expression. In the brain, they are involved in development and synaptic …

On non-detects in qPCR data

MN McCall, HR McMurray, H Land… - Bioinformatics, 2014 - academic.oup.com
Motivation: Quantitative real-time PCR (qPCR) is one of the most widely used methods to
measure gene expression. Despite extensive research in qPCR laboratory protocols …

Measurements methods for the development of MicroRNA-based tests for cancer diagnosis

F Precazzini, S Detassis, AS Imperatori… - International journal of …, 2021 - mdpi.com
Studies investigating microRNAs as potential biomarkers for cancer, immune-related
diseases, or cardiac pathogenic diseases, among others, have exponentially increased in …

Germline variants in tumor suppressor FBXW7 lead to impaired ubiquitination and a neurodevelopmental syndrome

SEM Stephenson, G Costain, LER Blok, MA Silk… - The American Journal of …, 2022 - cell.com
Neurodevelopmental disorders are highly heterogenous conditions resulting from
abnormalities of brain architecture and/or function. FBXW7 (F-box and WD-repeat-domain …

[HTML][HTML] MicroRNA signatures in vitreous humour and plasma of patients with exudative AMD

C Ménard, FA Rezende, K Miloudi, A Wilson… - Oncotarget, 2016 - ncbi.nlm.nih.gov
Age-related macular degeneration (AMD) is a leading cause of blindness worldwide
affecting individuals over the age of 50. The neovascular form (NV AMD) is characterized by …

MicroRNA-365 inhibits growth, invasion and metastasis of malignant melanoma by targeting NRP1 expression

J Bai, Z Zhang, X Li, H Liu - Cancer Biomarkers, 2015 - content.iospress.com
OBJECTIVE: The role of miR-365 in cancer cells seemed controversial in previous studies.
We thereby in this article aimed to define the role of miR-365 in malignant melanoma (MM) …

The choice of endogenous controls in exosomal microRNA assessments from biofluids

G Occhipinti, M Giulietti, G Principato, F Piva - Tumor Biology, 2016 - Springer
The assessment of differentially expressed microRNAs in patients and healthy controls is
important to identify potential tumor biomarkers. Recently, it has been shown that the …