[HTML][HTML] Single-molecule localization microscopy
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …
techniques that dramatically improve spatial resolution over standard, diffraction-limited …
Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels
JB Grimm, LD Lavis - Nature Methods, 2022 - nature.com
The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …
development of new microscopes, fluorescent labels and analysis techniques has pushed …
[HTML][HTML] The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation
K Cosentino, V Hertlein, A Jenner, T Dellmann… - Molecular cell, 2022 - cell.com
BAX and BAK are key apoptosis regulators that mediate the decisive step of mitochondrial
outer membrane permeabilization. However, the mechanism by which they assemble the …
outer membrane permeabilization. However, the mechanism by which they assemble the …
MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells
KC Gwosch, JK Pape, F Balzarotti, P Hoess… - Nature …, 2020 - nature.com
The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …
[HTML][HTML] Super-resolution microscopy for structural cell biology
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …
[HTML][HTML] MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope
R Schmidt, T Weihs, CA Wurm, I Jansen… - Nature …, 2021 - nature.com
The recently introduced minimal photon fluxes (MINFLUX) concept pushed the resolution of
fluorescence microscopy to molecular dimensions. Initial demonstrations relied on custom …
fluorescence microscopy to molecular dimensions. Initial demonstrations relied on custom …
[HTML][HTML] Dna-paint minflux nanoscopy
LM Ostersehlt, DC Jans, A Wittek, J Keller-Findeisen… - Nature …, 2022 - nature.com
MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient
fluorophore localizations, has brought about three-dimensional resolution at nanometer …
fluorophore localizations, has brought about three-dimensional resolution at nanometer …
Deep learning enables fast and dense single-molecule localization with high accuracy
Single-molecule localization microscopy (SMLM) has had remarkable success in imaging
cellular structures with nanometer resolution, but standard analysis algorithms require …
cellular structures with nanometer resolution, but standard analysis algorithms require …
Direct observation of motor protein stepping in living cells using MINFLUX
Dynamic measurements of molecular machines can provide invaluable insights into their
mechanism, but these measurements have been challenging in living cells. Here, we …
mechanism, but these measurements have been challenging in living cells. Here, we …
Field-dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging
Single-molecule localization microscopy in a typical wide-field setup has been widely used
for investigating subcellular structures with super resolution; however, field-dependent …
for investigating subcellular structures with super resolution; however, field-dependent …