Multiphoton microscopy has enabled unprecedented dynamic exploration in living
organisms. A significant challenge in biological research is the dynamic imaging of features …

▪ Abstract Two-photon fluorescence microscopy is one of the most important recent
inventions in biological imaging. This technology enables noninvasive study of biological …

The ability to perform optical sectioning is one of the great advantages of laser-scanning
microscopy. This introduces, however, a number of difficulties due to the scanning process …

We study and demonstrate the technique of simultaneous spatial and temporal focusing of
femtosecond pulses, with the aim to improve the signal-to-background ratio in multiphoton …

We present a real-time, direct-view multiphoton excitation fluorescence microscope that
provides three-dimensional imaging at high resolution. Using a rotating microlens disk, we …

Two-photon microscopy is a mainstay technique for imaging in scattering media and
normally provides frame-acquisition rates of~ 10–30 Hz. To track high-speed phenomena …

Since the work of Golgi and Cajal, light microscopy has remained a key tool for
neuroscientists to observe cellular properties. Ongoing advances have enabled new …

We report a substantial signal gain in fluorescence microscopy by ensuring that transient
molecular dark states with lifetimes> 1 μs, such as the triplet state relax between two …

This article reviews the latest instrumentation used in high resolution nonlinear microscopy
and techniques for the temporal and spatial calibration of this instrumentation. This includes …

Abstract A video-rate (30 frames/s) scanning two-photon excitation microscope has been
successfully tested. The microscope, based on a Nikon RCM 8000, incorporates a …