Deletions associated with the repair of DNA double‐strand breaks is a source of genetic
alternation and a recognized source of disease‐causing mutagenesis. Theta‐mediated end …

DNA double-strand breaks (DSBs) must be properly repaired within diverse chromatin
domains to maintain genome stability. Whereas euchromatin has an open structure and is …

Undesired on-and off-target effects of CRISPR-Cas nucleases remain a challenge in
genome editing. While the use of Cas9 nickases has been shown to minimize off-target …

Small tandem DNA duplications in the range of 15 to 300 base‐pairs play an important role
in the aetiology of human disease and contribute to genome diversity. Here, we discuss …

Gene editing through repair of CRISPR-Cas9-induced chromosomal breaks offers a means
to correct a wide range of genetic defects. Directing repair to produce desirable outcomes by …

Technologies to study DNA double-strand break (DSB) repair have traditionally mostly relied
on fluorescence read-outs, either by microscopy or flow cytometry. The advent of high …

With the advancement of CRISPR technologies, a comprehensive understanding of repair
mechanisms following double‐strand break (DSB) formation is important for improving the …

A practical and powerful approach for genome editing in plants is delivery of CRISPR
reagents via Agrobacterium tumefaciens transformation. The double-strand break (DSB) …

CRISPR-mediated endogenous tagging, utilizing the homology-directed repair (HDR) of
DNA double-strand breaks (DSBs) with exogenously incorporated donor DNA, is a powerful …

DNA double-strand breaks (DSBs) are predominantly resolved by the error-prone non-
homologous end-joining (NHEJ) or high-fidelity homologous recombination (HR) DNA …