Light-sheets and smart microscopy, an exciting future is dawning

S Daetwyler, RP Fiolka - Communications biology, 2023 - nature.com
Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Expansion microscopy: A chemical approach for super-resolution microscopy

Y Zhuang, X Shi - Current opinion in structural biology, 2023 - Elsevier
Super-resolution microscopy is a series of imaging techniques that bypass the diffraction
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …

Expansion microscopy of apicomplexan parasites

B Liffner, S Absalon - Molecular Microbiology, 2024 - Wiley Online Library
Apicomplexan parasites comprise significant pathogens of humans, livestock and wildlife,
but also represent a diverse group of eukaryotes with interesting and unique cell biology …

Seeing plants as never before

L Borisjuk, P Horn, K Chapman, PM Jakob… - New …, 2023 - Wiley Online Library
Imaging has long supported our ability to understand the inner life of plants, their
development, and response to a dynamic environment. While optical microscopy remains …

Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs

Y Cui, G Yang, DR Goodwin, CH O'Flanagan, A Sinha… - Plos one, 2023 - journals.plos.org
Expansion microscopy (ExM), by physically enlarging specimens in an isotropic fashion,
enables nanoimaging on standard light microscopes. Key to existing ExM protocols is the …

MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissues

Z Cheng, C Stefani, T Skillman, A Klimas… - Advanced …, 2023 - Wiley Online Library
Super‐resolution optical imaging tools are crucial in microbiology to understand the
complex structures and behavior of microorganisms such as bacteria, fungi, and viruses …

Next-Generation Sequencing-Based Spatial Transcriptomics: A Perspective from Barcoding Chemistry

W Shi, J Zhang, S Huang, Q Fan, J Cao, J Zeng, L Wu… - JACS Au, 2024 - ACS Publications
Gene expression profiling of tissue cells with spatial context is in high demand to reveal cell
types, locations, and intercellular or molecular interactions for physiological and …

Cellular cartography: Towards an atlas of the neuronal microtubule cytoskeleton

MK Iwanski, LC Kapitein - Frontiers in Cell and Developmental …, 2023 - frontiersin.org
Microtubules, one of the major components of the cytoskeleton, play a crucial role during
many aspects of neuronal development and function, such as neuronal polarization and …

Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging

UTT Wang, X Tian, YH Liou, SP Lee, HT Hu, CH Lu… - Scientific Reports, 2023 - nature.com
Expansion microscopy, whereby the relative positions of biomolecules are physically
increased via hydrogel expansion, can be used to reveal ultrafine structures of cells under a …