Light-sheets and smart microscopy, an exciting future is dawning
S Daetwyler, RP Fiolka - Communications biology, 2023 - nature.com
Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …
quantitatively measure biological processes rapidly and over long time periods. In this …
Current progress in expansion microscopy: chemical strategies and applications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …
visualization of biological targets at nanoscale resolution on conventional fluorescence …
Expansion microscopy: A chemical approach for super-resolution microscopy
Super-resolution microscopy is a series of imaging techniques that bypass the diffraction
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …
Expansion microscopy of apicomplexan parasites
Apicomplexan parasites comprise significant pathogens of humans, livestock and wildlife,
but also represent a diverse group of eukaryotes with interesting and unique cell biology …
but also represent a diverse group of eukaryotes with interesting and unique cell biology …
Seeing plants as never before
Imaging has long supported our ability to understand the inner life of plants, their
development, and response to a dynamic environment. While optical microscopy remains …
development, and response to a dynamic environment. While optical microscopy remains …
Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs
Expansion microscopy (ExM), by physically enlarging specimens in an isotropic fashion,
enables nanoimaging on standard light microscopes. Key to existing ExM protocols is the …
enables nanoimaging on standard light microscopes. Key to existing ExM protocols is the …
MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissues
Super‐resolution optical imaging tools are crucial in microbiology to understand the
complex structures and behavior of microorganisms such as bacteria, fungi, and viruses …
complex structures and behavior of microorganisms such as bacteria, fungi, and viruses …
Next-Generation Sequencing-Based Spatial Transcriptomics: A Perspective from Barcoding Chemistry
Gene expression profiling of tissue cells with spatial context is in high demand to reveal cell
types, locations, and intercellular or molecular interactions for physiological and …
types, locations, and intercellular or molecular interactions for physiological and …
Cellular cartography: Towards an atlas of the neuronal microtubule cytoskeleton
MK Iwanski, LC Kapitein - Frontiers in Cell and Developmental …, 2023 - frontiersin.org
Microtubules, one of the major components of the cytoskeleton, play a crucial role during
many aspects of neuronal development and function, such as neuronal polarization and …
many aspects of neuronal development and function, such as neuronal polarization and …
Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging
UTT Wang, X Tian, YH Liou, SP Lee, HT Hu, CH Lu… - Scientific Reports, 2023 - nature.com
Expansion microscopy, whereby the relative positions of biomolecules are physically
increased via hydrogel expansion, can be used to reveal ultrafine structures of cells under a …
increased via hydrogel expansion, can be used to reveal ultrafine structures of cells under a …