Methods of measuring enzyme activity ex vivo and in vivo
Enzymes catalyze a variety of biochemical reactions in the body and, in conjunction with
transporters and receptors, control virtually all physiological processes. There is great value …
transporters and receptors, control virtually all physiological processes. There is great value …
Electroosmotic perfusion, external microdialysis: Simulation and experiment
Information about the rates of hydrolysis of neuropeptides by extracellular peptidases can
lead to a quantitative understanding of how the steady-state and transient concentrations of …
lead to a quantitative understanding of how the steady-state and transient concentrations of …
Electrokinetic convection-enhanced delivery of solutes to the brain
Pressure-induced infusion of solutions into brain tissue is used both in research and in
medicine. In medicine, convection enhanced delivery (CED) may be used to deliver agents …
medicine. In medicine, convection enhanced delivery (CED) may be used to deliver agents …
Temperature-based on-column solute focusing in capillary liquid chromatography reduces peak broadening from pre-column dispersion and volume overload when …
SR Groskreutz, AR Horner, SG Weber - Journal of Chromatography A, 2015 - Elsevier
On-column focusing is essential for satisfactory performance using capillary scale columns.
On-column focusing results from generating transient conditions at the head of the column …
On-column focusing results from generating transient conditions at the head of the column …
Electroosmotic perfusion–microdialysis probe created by direct laser writing for quantitative assessment of leucine enkephalin hydrolysis by insulin-regulated …
RE Wilson, A Jaquins-Gerstl, J Chen, M Rerick… - Analytical …, 2020 - ACS Publications
There are many processes that actively alter the concentrations of solutes in the extracellular
space. Enzymatic reactions, either by soluble enzymes or membrane-bound ectoenzymes …
space. Enzymatic reactions, either by soluble enzymes or membrane-bound ectoenzymes …
Electrokinetic infusions into hydrogels and brain tissue: Control of direction and magnitude of solute delivery
Background Delivering solutes to a particular region of the brain is currently achieved by
iontophoresis for very small volumes and by diffusion from a microdialysis probe for larger …
iontophoresis for very small volumes and by diffusion from a microdialysis probe for larger …
Electroosmosis based novel treatment approach for cerebral edema
Objective: Cerebral edema characterized as an abnormal accumulation of interstitial fluid
has not been treated effectively. We propose a novel edema treatment approach to drive …
has not been treated effectively. We propose a novel edema treatment approach to drive …
Higher aminopeptidase activity determined by electroosmotic push–pull perfusion contributes to selective vulnerability of the hippocampal CA1 region to oxygen …
It has been known for over a century that the hippocampus, the center for learning and
memory in the brain, is selectively vulnerable to ischemic damage, with the CA1 being more …
memory in the brain, is selectively vulnerable to ischemic damage, with the CA1 being more …
Development of μ-Low-Flow-Push–Pull Perfusion Probes for Ex Vivo Sampling from Mouse Hippocampal Tissue Slices
MR Cabay, A McRay, DE Featherstone… - ACS Chemical …, 2018 - ACS Publications
This work demonstrates a reduced tip μ-low-flow-push–pull perfusion technique for ex vivo
sampling of the extracellular space of mouse hippocampal brain slices. Concentric fused …
sampling of the extracellular space of mouse hippocampal brain slices. Concentric fused …
Numerical modeling of electroosmotic push–pull perfusion and assessment of its application to quantitative determination of enzymatic activity in the extracellular …
Many sampling methods have been developed to measure the extracellular concentrations
of solutes in the extracellular space of mammalian tissue, eg, brain. However, few have …
of solutes in the extracellular space of mammalian tissue, eg, brain. However, few have …