Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture

H Huang, Y Yu, Y Hu, X He, OB Usta, ML Yarmush - Lab on a Chip, 2017 - pubs.rsc.org
Hydrogel microcapsules provide miniaturized and biocompatible niches for three-
dimensional (3D) in vitro cell culture. They can be easily generated by droplet-based …

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

P Zhang, N Shao, L Qin - Advanced Materials, 2021 - Wiley Online Library
Cell‐based living materials, including single cells, cell‐laden fibers, cell sheets, organoids,
and organs, have attracted intensive interests owing to their widespread applications in …

[HTML][HTML] A practical guide to microfabrication and patterning of hydrogels for biomimetic cell culture scaffolds

M Tenje, F Cantoni, AMP Hernández, SS Searle… - Organs-on-a-Chip, 2020 - Elsevier
This review article describes microfabrication techniques to define chemical, mechanical
and structural patterns in hydrogels and how these can be used to prepare in vivo like, ie …

Stem cell microencapsulation for phenotypic control, bioprocessing, and transplantation

JL Wilson, TC McDevitt - Biotechnology and bioengineering, 2013 - Wiley Online Library
Cell microencapsulation has been utilized for decades as a means to shield cells from the
external environment while simultaneously permitting transport of oxygen, nutrients, and …

Microfluidics-based fabrication of cell-laden microgels

MGA Mohamed, P Ambhorkar, R Samanipour… - …, 2020 - pubs.aip.org
Microfluidic principles have been extensively utilized as powerful tools to fabricate controlled
monodisperse cell-laden hydrogel microdroplets for various biological applications …

Bottom‐up engineering of well‐defined 3D microtissues using microplatforms and biomedical applications

GH Lee, JS Lee, X Wang… - Advanced healthcare …, 2016 - Wiley Online Library
During the last decades, the engineering of well‐defined 3D tissues has attracted great
attention because it provides in vivo mimicking environment and can be a building block for …

Long‐term perfusion culture of monoclonal embryonic stem cells in 3D hydrogel beads for continuous optical analysis of differentiation

H Kleine‐Brüggeney, LD Van Vliet, C Mulas, F Gielen… - Small, 2019 - Wiley Online Library
Developmental cell biology requires technologies in which the fate of single cells is followed
over extended time periods, to monitor and understand the processes of self‐renewal …

Microfluidic production of single micrometer-sized hydrogel beads utilizing droplet dissolution in a polar solvent

S Sugaya, M Yamada, A Hori, M Seki - Biomicrofluidics, 2013 - pubs.aip.org
In this study, a microfluidic process is proposed for preparing monodisperse micrometer-
sized hydrogel beads. This process utilizes non-equilibrium aqueous droplets formed in a …

Patterning of microspheres and microbubbles in an acoustic tweezers

AL Bernassau, PGA MacPherson, J Beeley… - Biomedical …, 2013 - Springer
We describe the construction of an ultrasonic device capable of micro-patterning a range of
microscopic particles for bioengineering applications such as targeted drug delivery. The …

Droplet trapping and fast acoustic release in a multi-height device with steady-state flow

RW Rambach, K Linder, M Heymann, T Franke - Lab on a Chip, 2017 - pubs.rsc.org
We demonstrate a novel multilayer polydimethylsiloxane (PDMS) device for selective
storage and release of single emulsion droplets. Drops are captured in a microchannel …