Gonadal development is a complex process that involves sex determination followed by
divergent maturation into either testes or ovaries. Historically, limited tissue accessibility, a …

Human pluripotent stem cells harbor the capacity to differentiate into cells from the three
embryonic germ layers, and this ability grants them a central role in modeling human …

In this review, advances in the understanding of epigenetic reprogramming from fertilization
to the development of primordial germline cells in a mouse and human embryo are …

Totipotency is the ability of a single cell to give rise to all of the differentiated cell types that
build the conceptus, yet how to capture this property in vitro remains incompletely …

Cell differentiation typically occurs with concomitant shape transitions to enable specialized
functions. To adopt a different shape, cells need to change the mechanical properties of their …

During embryonic development, naive pluripotent epiblast cells transit to a formative state.
The formative epiblast cells form a polarized epithelium, exhibit distinct transcriptional and …

We present scMAGeCK, a computational framework to identify genomic elements
associated with multiple expression-based phenotypes in CRISPR/Cas9 functional …

Early mammalian development entails genome-wide epigenome remodeling, including
DNA methylation erasure and reacquisition, which facilitates developmental competence. To …

As embryonic stem cells (ESCs) transition from naive to primed pluripotency during early
mammalian development, they acquire high DNA methylation levels. During this transition …

The generation of genomically stable and functional oocytes has great potential for
preserving fertility and restoring ovarian function. It remains elusive whether functional …