The CRISPR‐Cas‐based genome editing field in plants is expanding rapidly. Editing plant
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …

Common wheat (Triticum aestivum) is one of the most widely cultivated and consumed crops
globally. In the face of limited arable land and climate changes, it is a great challenge to …

Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9),
sourced from a human pathogen, is the most widely used. Here, through in silico data …

Site-specific recombinases such as the Cre-LoxP system are routinely used for genome
engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the …

Summary The Er Cas12a nuclease, also known as MAD7, is part of a CRISPR/Cas system
from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31 …

Background CRISPR/Cas-derived base editor enables precise editing of target sites and
has been widely used for basic research and crop genetic improvement. However, the …

ABSTRACT CRISPR/Cas9 gene editing system is recently developed robust genome
editing technology for accelerating plant breeding. Various modifications of this editing …

Following recent developments and refinement, CRISPR-Cas9 gene-editing technology has
become increasingly mature and is being widely used for crop improvement. The application …

Abstract Background CRISPR Cas9 and Cas12a are the two most frequently used
programmable nucleases reported in plant systems. There is now a wide range of …

Transgene‐free genome editing based on clustered regularly interspaced short palindromic
repeats (CRISPR) technology is key to achieving genetic engineering in microalgae for …