[HTML][HTML] Strategies for precise gene edits in mammalian cells
KM Fichter, T Setayesh, P Malik - Molecular Therapy-Nucleic Acids, 2023 - cell.com
CRISPR-Cas technologies have the potential to revolutionize genetic medicine. However,
work is still needed to make this technology clinically efficient for gene correction. A barrier …
work is still needed to make this technology clinically efficient for gene correction. A barrier …
Prime editing: Mechanism insight and recent applications in plants
Prime editing (PE) technology utilizes an extended prime editing guide RNA (pegRNA) to
direct a fusion peptide consisting of nCas9 (H840) and reverse transcriptase (RT) to a …
direct a fusion peptide consisting of nCas9 (H840) and reverse transcriptase (RT) to a …
Patterns of recombination in snakes reveal a tug-of-war between PRDM9 and promoter-like features
In some mammals, notably humans, recombination occurs almost exclusively where the
protein PRDM9 binds, whereas in vertebrates lacking an intact PRDM9, such as birds and …
protein PRDM9 binds, whereas in vertebrates lacking an intact PRDM9, such as birds and …
[HTML][HTML] Recursive Editing improves homology-directed repair through retargeting of undesired outcomes
CRISPR-Cas induced homology-directed repair (HDR) enables the installation of a broad
range of precise genomic modifications from an exogenous donor template. However …
range of precise genomic modifications from an exogenous donor template. However …
Machine learning prediction of prime editing efficiency across diverse chromatin contexts
The success of prime editing depends on the prime editing guide RNA (pegRNA) design
and target locus. Here, we developed machine learning models that reliably predict prime …
and target locus. Here, we developed machine learning models that reliably predict prime …
A landing pad system for multicopy gene integration in Issatchenkia orientalis
The robust nature of the non-conventional yeast Issatchenkia orientalis allows it to grow
under highly acidic conditions and therefore, has gained increasing interest in producing …
under highly acidic conditions and therefore, has gained increasing interest in producing …
[HTML][HTML] CRISPR-Cas9 Direct Fusions for Improved Genome Editing via Enhanced Homologous Recombination
T Tabassum, G Pietrogrande, M Healy… - International Journal of …, 2023 - mdpi.com
DNA repair in mammalian cells involves the coordinated action of a range of complex
cellular repair machinery. Our understanding of these DNA repair processes has advanced …
cellular repair machinery. Our understanding of these DNA repair processes has advanced …
[HTML][HTML] Lenvatinib resistance mechanism and potential ways to conquer
W Bo, Y Chen - Frontiers in Pharmacology, 2023 - frontiersin.org
Lenvatinib (LVN) has been appoved to treat advanced renal cell carcinoma, differentiated
thyroid carcinoma, hepatocellular carcinoma. Further other cancer types also have been …
thyroid carcinoma, hepatocellular carcinoma. Further other cancer types also have been …
CRISPR-based epigenome editing: mechanisms and applications
SM Fadul, A Arshad, R Mehmood - Epigenomics, 2023 - Taylor & Francis
Epigenomic anomalies contribute significantly to the development of numerous human
disorders. The development of epigenetic research tools is essential for understanding how …
disorders. The development of epigenetic research tools is essential for understanding how …
Clustered regularly interspaced short palindromic repeats and clustered regularly Interspaced Short Palindromic Repeats–Associated protein 9 system: factors …
J Li, C Tang, G Liang, H Tian, G Lai, Y Wu… - Human Gene …, 2023 - liebertpub.com
The clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated
(Cas) system is a powerful genomic DNA editing tool. The increased applications of gene …
(Cas) system is a powerful genomic DNA editing tool. The increased applications of gene …