Cross-linked enzyme aggregates immobilization: Preparation, characterization, and applications
N Chen, B Chang, N Shi, W Yan, F Lu… - Critical Reviews in …, 2023 - Taylor & Francis
Enzymes are commonly used as biocatalysts for various biological and chemical processes.
However, some major drawbacks of free enzymes (eg poor reusability and instability) …
However, some major drawbacks of free enzymes (eg poor reusability and instability) …
From enzyme stability to enzymatic bioelectrode stabilization processes
C Beaufils, HM Man, A de Poulpiquet, I Mazurenko… - Catalysts, 2021 - mdpi.com
Bioelectrocatalysis using redox enzymes appears as a sustainable way for biosensing,
electricity production, or biosynthesis of fine products. Despite advances in the knowledge of …
electricity production, or biosynthesis of fine products. Despite advances in the knowledge of …
Preparation of cross-linked enzyme aggregates of lipase from Aspergillus niger: process optimization, characterization, stability, and application for epoxidation of …
Cross-linked enzyme aggregates (CLEAs) of lipase were prepared after fractional
precipitation with 40–50% ammonium sulfate and then cross-linking with glutaraldehyde …
precipitation with 40–50% ammonium sulfate and then cross-linking with glutaraldehyde …
Immobilization of lipase on silica nanoparticles by adsorption followed by glutaraldehyde cross-linking
J Qian, A Huang, H Zhu, J Ding, W Zhang… - Bioprocess and …, 2023 - Springer
In this study, Candida antarctica lipase B was immobilized on silica (SiO2) nanoparticles by
physical adsorption, and then cross-linked with glutaraldehyde (GA) to prepare cross-linked …
physical adsorption, and then cross-linked with glutaraldehyde (GA) to prepare cross-linked …
Cross-linked enzyme aggregates (CLEAs) of cellulase with improved catalytic activity, adaptability and reusability
T Li, X Gong, G Yang, Q Li, J Huang, N Zhou… - Bioprocess and …, 2022 - Springer
In this study, cross-linked cellulase aggregates (C-CLEAs) were synthesized by precipitation
of cellulase with ammonium sulfate and then cross-linking with glutaraldehyde. The results …
of cellulase with ammonium sulfate and then cross-linking with glutaraldehyde. The results …
Cross-linked β-mannanase aggregates: preparation, characterization, and application for producing partially hydrolyzed guar gum
S Behera, MJ Dev, RS Singhal - Applied Biochemistry and Biotechnology, 2022 - Springer
Abstract β-Mannanase capable of hydrolyzing β-1, 4-linkages in guar gum was immobilized
as cross-linked enzyme aggregates (M-CLEAs). The aggregation and cross-linking process …
as cross-linked enzyme aggregates (M-CLEAs). The aggregation and cross-linking process …
Preparation, characterization and stability of cross linked nitrilase aggregates (nitrilase–CLEAs) for hydroxylation of 2-chloroisonicotinonitrile to 2-chloroisonicotinic …
Nitrilases capable of performing hydroxylation of 2-chloroisonicotinonitrile to 2-
chloroisonicotinic acid were screened, and ES-NIT-102 was the best nitrilase for said …
chloroisonicotinic acid were screened, and ES-NIT-102 was the best nitrilase for said …
An Overview of Crosslinked Enzyme Aggregates: Concept of Development and Trends of Applications
Enzymes are commonly used as biocatalysts for various biological and chemical processes
in industrial applications. However, their limited operational stability, catalytic efficiency, poor …
in industrial applications. However, their limited operational stability, catalytic efficiency, poor …
Enhanced biochemical characteristics of β-glucosidase via adsorption and cross-linked enzyme aggregate for rapid cellobiose hydrolysis
X Deng, T He, J Li, HL Duan, ZQ Zhang - Bioprocess and biosystems …, 2020 - Springer
With proper design, immobilization can be useful tool to improve the stability of enzymes,
and in certain cases even their activity, selectivity, productivity and economic viability. An …
and in certain cases even their activity, selectivity, productivity and economic viability. An …
Cross-linked enzyme aggregates of polyethylene terephthalate hydrolyse (PETase) from Ideonella sakaiensis for the improvement of plastic degradation
Polyethylene terephthalate (PET) is one of the most produced plastics globally and its
accumulation in the environment causes harm to the ecosystem. Polyethylene terephthalate …
accumulation in the environment causes harm to the ecosystem. Polyethylene terephthalate …