Spinach is an in vitro–selected RNA aptamer that binds a GFP-like ligand and activates its
green fluorescence. Spinach is thus an RNA analog of GFP and has potentially widespread …

Advances in our understanding of macromolecular structure come from experimental
methods, such as X-ray crystallography, and also computational analysis of the growing …

CRISPR adaptive immune systems protect bacteria from infections by deploying CRISPR
RNA (crRNA)-guided enzymes to recognize and cut foreign nucleic acids. Type VI-A …

Structures of riboswitch receptor domains bound to their effector have shown how
messenger RNAs recognize diverse small molecules, but mechanistic details linking the …

Group II introns are self-splicing ribozymes that share a reaction mechanism and a common
ancestor with the eukaryotic spliceosome, thereby providing a model system for …

In all domains of life, selenocysteine (Sec) is delivered to the ribosome by selenocysteine-
specific tRNA (tRNASec) with the help of a specialized translation factor, SelB in bacteria …

Branching is a critical step in RNA splicing that is essential for 5′ splice site selection.
Recent spliceosome structures have led to competing models for the recognition of the …

The Varkud satellite (VS) ribozyme mediates rolling-circle replication of a plasmid found in
the Neurospora mitochondrion. We report crystal structures of this ribozyme from …

Three-dimensional RNA models fitted into crystallographic density maps exhibit pervasive
conformational ambiguities, geometric errors and steric clashes. To address these problems …

Noncoding RNAs (ncRNAs) regulate gene expression in all organisms. Bacterial 6S RNAs
globally regulate transcription by binding RNA polymerase (RNAP) holoenzyme and …