Intracellular mRNA transport and localized translation

S Das, M Vera, V Gandin, RH Singer… - Nature reviews Molecular …, 2021 - nature.com
Fine-tuning cellular physiology in response to intracellular and environmental cues requires
precise temporal and spatial control of gene expression. High-resolution imaging …

[HTML][HTML] Cell fate decisions, transcription factors and signaling during early retinal development

R Diacou, P Nandigrami, A Fiser, W Liu… - Progress in retinal and …, 2022 - Elsevier
The development of the vertebrate eyes is a complex process starting from anterior-posterior
and dorso-ventral patterning of the anterior neural tube, resulting in the formation of the eye …

Mechanisms tailoring the expression of heat shock proteins to proteostasis challenges

LRA Boopathy, S Jacob-Tomas, C Alecki… - Journal of Biological …, 2022 - ASBMB
All cells possess an internal stress response to cope with environmental and
pathophysiological challenges. Upon stress, cells reprogram their molecular functions to …

A growing toolbox to image gene expression in single cells: sensitive approaches for demanding challenges

X Pichon, M Lagha, F Mueller, E Bertrand - Molecular cell, 2018 - cell.com
The spatiotemporal regulation of gene expression is key to many biological processes.
Recent imaging approaches opened exciting perspectives for understanding the intricate …

An improved imaging system that corrects MS2-induced RNA destabilization

W Li, A Maekiniemi, H Sato, C Osman, RH Singer - Nature methods, 2022 - nature.com
The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study
messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2 …

Live cell imaging of single RNA molecules with fluorogenic Mango II arrays

AD Cawte, PJ Unrau, DS Rueda - Nature communications, 2020 - nature.com
RNA molecules play vital roles in many cellular processes. Visualising their dynamics in live
cells at single-molecule resolution is essential to elucidate their role in RNA metabolism …

A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells

E Braselmann, AJ Wierzba, JT Polaski… - Nature chemical …, 2018 - nature.com
RNAs directly regulate a vast array of cellular processes, emphasizing the need for robust
approaches to fluorescently label and track RNAs in living cells. Here, we develop an RNA …

Illuminating RNA biology: tools for imaging RNA in live mammalian cells

E Braselmann, C Rathbun, EM Richards… - Cell chemical biology, 2020 - cell.com
The central dogma teaches us that DNA makes RNA, which in turn makes proteins, the main
building blocks of the cell. But this over simplified linear transmission of information …

The myonuclear domain in adult skeletal muscle fibres: past, present and future

JR Bagley, LT Denes, JJ McCarthy… - The Journal of …, 2023 - Wiley Online Library
Most cells in the body are mononuclear whereas skeletal muscle fibres are uniquely
multinuclear. The nuclei of muscle fibres (myonuclei) are usually situated peripherally which …

Significance of lncRNA abundance to function

I Grammatikakis, A Lal - Mammalian Genome, 2022 - Springer
Long noncoding RNAs (lncRNAs) have emerged as regulators of diverse cellular processes.
Although the vast majority of lncRNAs are expressed at lower levels compared to …