Dynamic nuclear polarization for sensitivity enhancement in biomolecular solid-state NMR

T Biedenbänder, V Aladin, S Saeidpour… - Chemical …, 2022 - ACS Publications
Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

Practical dissolution dynamic nuclear polarization

SJ Elliott, Q Stern, M Ceillier, T El Daraï… - Progress in Nuclear …, 2021 - Elsevier
This review article intends to provide insightful advice for dissolution-dynamic nuclear
polarization in the form of a practical handbook. The goal is to aid research groups to …

Ultrafast bioorthogonal spin-labeling and distance measurements in mammalian cells using small, genetically encoded tetrazine amino acids

S Jana, EGB Evans, HS Jang, S Zhang… - Journal of the …, 2023 - ACS Publications
Site-directed spin-labeling (SDSL)─ in combination with double electron–electron
resonance (DEER) spectroscopy─ has emerged as a powerful technique for determining …

Targeted DNP for biomolecular solid-state NMR

D Gauto, O Dakhlaoui, I Marin-Montesinos… - Chemical …, 2021 - pubs.rsc.org
High-field dynamic nuclear polarization is revolutionizing the scope of solid-state NMR with
new applications in surface chemistry, materials science and structural biology. In this …

Dynamic nuclear polarization nuclear magnetic resonance in human cells using fluorescent polarizing agents

BJ Albert, C Gao, EL Sesti, EP Saliba, N Alaniva… - Biochemistry, 2018 - ACS Publications
Solid state nuclear magnetic resonance (NMR) enables atomic-resolution characterization
of the molecular structure and dynamics within complex heterogeneous samples, but it is …

19F Dynamic Nuclear Polarization at Fast Magic Angle Spinning for NMR of HIV-1 Capsid Protein Assemblies

M Lu, M Wang, IV Sergeyev, CM Quinn… - Journal of the …, 2019 - ACS Publications
We report remarkably high, up to 100-fold, signal enhancements in 19F dynamic nuclear
polarization (DNP) magic angle spinning (MAS) spectra at 14.1 T on HIV-1 capsid protein …

Recent advances in solid-state nuclear magnetic resonance spectroscopy of exotic nuclei

C Leroy, DL Bryce - Progress in Nuclear Magnetic Resonance …, 2018 - Elsevier
We present a review of recent advances in solid-state nuclear magnetic resonance
(SSNMR) studies of exotic nuclei. Exotic nuclei may be spin-1/2 or quadrupolar, and …

Use of paramagnetic systems to speed-up NMR data acquisition and for structural and dynamic studies

V Kocman, GM Di Mauro, G Veglia… - Solid state nuclear …, 2019 - Elsevier
NMR spectroscopy is a powerful experimental technique to study biological systems at the
atomic resolution. However, its intrinsic low sensitivity results in long acquisition times that in …

MAS-DNP enhancements: hyperpolarization, depolarization, and absolute sensitivity

S Hediger, D Lee, F Mentink-Vigier… - Handbook of High …, 2019 - books.google.com
Since the earliest days of solid-state dynamic nuclear polarization (DNP) and the first
experiment by Carver and Slichter on lithium, 1 the ability of this technique to enhance the …