Bridging the gap: from 2D cell culture to 3D microengineered extracellular matrices
Historically the culture of mammalian cells in the laboratory has been performed on planar
substrates with media cocktails that are optimized to maintain phenotype. However, it is …
substrates with media cocktails that are optimized to maintain phenotype. However, it is …
Biomedical analysis of formalin-fixed, paraffin-embedded tissue samples: The Holy Grail for molecular diagnostics
More than a century ago in 1893, a revolutionary idea about fixing biological tissue
specimens was introduced by Ferdinand Blum, a German physician. Since then, a plethora …
specimens was introduced by Ferdinand Blum, a German physician. Since then, a plethora …
Microscopy with ultraviolet surface excitation for rapid slide-free histology
F Fereidouni, ZT Harmany, M Tian, A Todd… - Nature biomedical …, 2017 - nature.com
Histological examination of tissues is central to the diagnosis and management of
neoplasms and many other diseases and is a foundational technique for preclinical and …
neoplasms and many other diseases and is a foundational technique for preclinical and …
Quantitative profiling of the human substantia nigra proteome from laser-capture microdissected FFPE tissue
E Griesser, H Wyatt, S Ten Have, B Stierstorfer… - Molecular & Cellular …, 2020 - ASBMB
Laser-capture microdissection (LCM) allows the visualization and isolation of
morphologically distinct subpopulations of cells from heterogeneous tissue specimens. In …
morphologically distinct subpopulations of cells from heterogeneous tissue specimens. In …
Brain banking for neurological disorders
Brain banks are used to gather, store, and provide human brain tissue for research and have
been fundamental to improving our knowledge of the brain in health and disease. To …
been fundamental to improving our knowledge of the brain in health and disease. To …
Proteomics and metabolomics for AKI diagnosis
D Marx, J Metzger, M Pejchinovski, RB Gil… - Seminars in …, 2018 - Elsevier
Acute kidney injury (AKI) is a severe and frequent condition in hospitalized patients.
Currently, no efficient therapy of AKI is available. Therefore, efforts focus on early prevention …
Currently, no efficient therapy of AKI is available. Therefore, efforts focus on early prevention …
Tissue proteomics for the next decade? Towards a molecular dimension in histology
R Longuespée, M Fléron, C Pottier… - OMICS: A Journal of …, 2014 - liebertpub.com
The concept of tissues appeared more than 200 years ago, since textures and attendant
differences were described within the whole organism components. Instrumental …
differences were described within the whole organism components. Instrumental …
Proteomic analysis of neurons microdissected from formalin-fixed, paraffin-embedded Alzheimer's disease brain tissue
The vast majority of human tissue specimens are formalin-fixed, paraffin embedded (FFPE)
archival samples, making this type of tissue a potential gold mine for medical research. It is …
archival samples, making this type of tissue a potential gold mine for medical research. It is …
The response to neoadjuvant chemoradiotherapy with 5-fluorouracil in locally advanced rectal cancer patients: a predictive proteomic signature
A Chauvin, CS Wang, S Geha, P Garde-Granger… - Clinical proteomics, 2018 - Springer
Background Colorectal cancer is the third most common and the fourth most lethal cancer in
the world. In the majority of cases, patients are diagnosed at an advanced stage or even …
the world. In the majority of cases, patients are diagnosed at an advanced stage or even …
Critical comparison of sample preparation strategies for shotgun proteomic analysis of formalin-fixed, paraffin-embedded samples: insights from liver tissue
Background The growing field of formalin-fixed paraffin-embedded (FFPE) tissue proteomics
holds promise for improving translational research. Direct tissue trypsinization (DT) and …
holds promise for improving translational research. Direct tissue trypsinization (DT) and …