Growth, replication and division enable evolution of coacervate protocells

AD Slootbeek, MHI van Haren, IBA Smokers… - Chemical …, 2022 - pubs.rsc.org
Living and proliferating cells undergo repeated cycles of growth, replication and division, all
orchestrated by complex molecular networks. How a minimal cell cycle emerged and helped …

Bio‐inspired functional coacervates: Special Issue: Emerging Investigators

S Chen, Q Guo, J Yu - Aggregate, 2022 - Wiley Online Library
Many functional coacervates have been identified in biological systems, which have
attracted widespread interest. Coacervation is a liquid–liquid phase separation (LLPS) …

Biomolecular condensates create phospholipid-enriched microenvironments

JG Dumelie, Q Chen, D Miller, N Attarwala… - Nature Chemical …, 2024 - nature.com
Proteins and RNA can phase separate from the aqueous cellular environment to form
subcellular compartments called condensates. This process results in a protein–RNA …

Ribozyme activity modulates the physical properties of RNA–peptide coacervates

KK Le Vay, E Salibi, B Ghosh, TYD Tang, H Mutschler - Elife, 2023 - elifesciences.org
Condensed coacervate phases are now understood to be important features of modern cell
biology, as well as valuable protocellular models in origin-of-life studies and synthetic …

Complex Coacervates as a Promising Vehicle for mRNA Delivery: A Comprehensive Review of Recent Advances and Challenges

C Forenzo, J Larsen - Molecular Pharmaceutics, 2023 - ACS Publications
Messenger RNA (mRNA)-based therapies have gained significant attention, following the
successful deployment of mRNA-based COVID-19 vaccines. Compared with traditional …

Hybrid Protocells Based on Coacervate‐Templated Fatty Acid Vesicles Combine Improved Membrane Stability with Functional Interior Protocytoplasm

J Lee, F Pir Cakmak, R Booth, CD Keating - Small, 2024 - Wiley Online Library
Prebiotically‐plausible compartmentalization mechanisms include membrane vesicles
formed by amphiphile self‐assembly and coacervate droplets formed by liquid–liquid phase …

RNA folding studies inside peptide-rich droplets reveal roles of modified nucleosides at the origin of life

MCO Meyer, R Yamagami, S Choi, CD Keating… - Science …, 2023 - science.org
Compartmentalization of RNA in biopolymer-rich membraneless organelles is now
understood to be pervasive and critical for the function of extant biology and has been …

The chromatin-associated lncREST ensures effective replication stress response by promoting the assembly of fork signaling factors

L Statello, JM Fernandez-Justel, J González… - Nature …, 2024 - nature.com
Besides the well-characterized protein network involved in the replication stress response,
several regulatory RNAs have been shown to play a role in this critical process. However, it …

Charge-Patterned Disordered Peptides Tune Intracellular Phase Separation in Bacteria

J Liao, V Yeong, AC Obermeyer - ACS Synthetic Biology, 2024 - ACS Publications
Subcellular phase-separated compartments, known as biomolecular condensates, play an
important role in the spatiotemporal organization of cells. To understand the sequence …

Aging RNA granule dynamics in neurodegeneration

K Rhine, N Al-Azzam, T Yu, GW Yeo - Frontiers in Molecular …, 2022 - frontiersin.org
Disordered RNA-binding proteins and repetitive RNA sequences are the main genetic
causes of several neurodegenerative diseases, including amyotrophic lateral sclerosis and …