Macromolecular crowding affects the activity of proteins and functional macromolecular
complexes in all cells, including bacteria. Crowding, together with physicochemical …

There has been increasing interest in recent years in describing the lateral organization of
membranes and the formation of membrane domains. Much of the focus in this area has …

More than 50 years have passed since the presentation of the Replicon Model which states
that a positively acting initiator interacts with a specific site on a circular chromosome …

The bacterium Escherichia coli initiates replication once per cell cycle at a precise volume
per origin and adds an on average constant volume between successive initiation events …

All cellular organisms and many viruses rely on large, multi-subunit molecular machines,
termed replisomes, to ensure that genetic material is accurately duplicated for transmission …

The DnaA protein has long been considered to play the key role in the initiation of
chromosome replication in modern bacteria. Many questions about this role, however …

Bacterial conjugation is the main mechanism responsible for the dissemination of antibiotic
resistance genes. Hence, the search for specific conjugation inhibitors is paramount in the …

Non-essential extra-chromosomal DNA elements such as plasmids are responsible for their
own propagation in dividing host cells, and one means to ensure this is to carry a miniature …

Anionic (ie., acidic) phospholipids such as phosphotidylglycerol (PG) and cardiolipin (CL),
participate in several cellular functions. Here we review intriguing in vitro and in vivo …

The Escherichia coli chromosomal origin consists of a duplex-unwinding region and a
region bearing a DNA-bending protein, IHF-binding site, and clusters of binding sites for the …