One of the major barriers to the use of enzymes in industrial biotechnology is their
insufficient stability under processing conditions. The use of organic solvent systems instead …

Nowadays, enzymes can be efficiently identified and screened from metagenomic resources
or mutant libraries. A set of a few hundred new enzymes can be found using a simple …

The abilities of enzymes to catalyze reactions in nonnatural environments of organic
solvents have opened new opportunities for enzyme-based industrial processes. However …

The long-established use of enzymes for food processing and product formulation has
resulted in an increased enzyme market compounding to 7.0% annual growth rate …

One clone (Lip906) exhibiting lipase activity was screened from a metagenomic library by
using a medium containing tricaprylin. A novel lipase gene from the inserted fragment of …

A novel cold-active true lipase from Pseudomonas sp. KE38 was cloned, sequencing and
expressed in E. coli by degenerate PCR and genome walking technique. The open reading …

Extreme environments are the main source of industrially suitable biocatalysts. The non-
cultivable approach of searching enzymes is known to provide ample scope to accomplish …

微生物脂肪酶是商品化脂肪酶的主要来源, 并广泛应用于诸多工业领域. 与其他微生物脂肪酶相
比, 细菌脂肪酶催化反应的类型更多, 活性更高, 稳定性更好, 其中又以假单胞菌属 …

Background Metagenomics is a useful tool in the search for new lipases that might have
characteristics that make them suitable for application in biocatalysis. This paper reports the …

A mesophilic Enterobacter sp. Bn12 producing an alkaline thermostable lipase was isolated
from soil in Tehran, Iran. The lipase gene (ELBn12) was identified from a genomic library …