Nucleic acids are excellent at recognizing complementary sequences through the formation
of Watson− Crick base pairs. Since the base pairing enables the highly selective …

Molecular interactions and reactions in living cells occur with high concentrations of
background molecules and ions. Many research studies have shown that intracellular …

The unconstrained genomic DNA of bacteria forms a coil, whose volume exceeds 1000
times the volume of the cell. Since prokaryotes lack a membrane-bound nucleus, in sharp …

DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is
essential for DNA replication and gene regulation. However, the density of supercoiling in …

We have examined the changes in physical properties of aqueous solutions of the plasmid
pUC18 that take place on the addition of the cationic oligopeptide penta-arginine. An …

This work addresses the question of the interplay of DNA demixing and supercoiling in
bacterial cells. Demixing of DNA from other globular macromolecules results from the overall …

The macromolecules of the bacterial cell occupy 20–40% of the total cytosol volume, and
crowded environments have long been known to compact and stabilize DNA. Nevertheless …

With a view to determining the distance between the two opposing duplexes in supercoiled
DNA, we have measured small angle neutron scattering from pHSG298 plasmid (2675 base …

We use lattice-Boltzmann molecular dynamics (LBMD) simulations to study the compression
of a confined polymer immersed in a fluid and pushed by a large spherical colloid with a …

In the presence of cationic ligands, DNA molecules can become aggregated into larger
particles in a process known as condensation. DNA condensates are of interest as models …