Microorganisms are a promising means to address many societal sustainability challenges
owing to their ability to thrive in diverse environments and interface with the microscale …

Abstract The SEVA platform (https://seva-plasmids. com) was launched one decade ago,
both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis …

Genome editing tools, through the disruption of an organism's native genetic material or the
introduction of non-native DNA, facilitate functional investigations to link genotypes to …

Living cells are exquisitely tuned to sense and respond to changes in their environment.
Repurposing these systems to create engineered biosensors has seen growing interest in …

Hazardous materials, such as heavy metals, are the major sources of health risk. Using
genetically modified organisms (GMOs) to dispose heavy metals has the advantages of …

Biological valorization of lignin, the second most abundant biopolymer on Earth, is an
indispensable sector to build a circular economy and net-zero future. However, lignin is …

Microbiology and synthetic biology depend on reverse genetic approaches to manipulate
bacterial genomes; however, existing methods require molecular biology to generate …

Controlled gene expression is crucial for engineering bacteria for basic and applied
research. Inducible systems enable tight regulation of expression, wherein a small-molecule …

The pGinger suite of expression plasmids comprises 43 plasmids that will enable precise
constitutive and inducible gene expression in a wide range of Gram-negative bacterial …

Staphylococcus aureus is an important clinical bacterium prevalent in human-associated
microbiomes and the cause of many diseases. However, S. aureus has been intractable to …