Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

I Gow, NC Smith, D Stark, J Ellis - Parasites & Vectors, 2022 - Springer
Leishmania infections span a range of clinical syndromes and impact humans from many
geographic foci, but primarily the world's poorest regions. Transmitted by the bite of a female …

[HTML][HTML] Leishmaniosis caused by Leishmania infantum in ferrets: update review

S Villanueva-Saz, J Giner, D Marteles, M Verde… - Veterinary and Animal …, 2022 - Elsevier
Leishmaniosis in domestic ferrets (Mustela putorius furo) is a disease caused by Leishmania
infantum, a parasite transmitted through the bite of an infected female phlebotomine sand fly …

Molecular diagnosis of leishmaniasis: quantification of parasite load by a real-time PCR assay with high sensitivity

G Castelli, F Bruno, S Reale, S Catanzaro, V Valenza… - Pathogens, 2021 - mdpi.com
Real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and
optimized to achieve a sensitivity of 1 parasite/mL. For this purpose, we cloned the …

High-resolution melting (HRM)-based detection of polymorphisms in the malic enzyme and glucose-6-phosphate isomerase genes for Leishmania infantum …

G Buffi, M Ceccarelli, A Diotallevi, M Abruzzese… - Parasites & …, 2023 - Springer
Background Leishmaniasis is a zoonotic disease endemic in the Mediterranean region
where Leishmania infantum is the causative agent of human and canine infection …

Exosome secretion by Leishmania infantum modulate the chemotactic behavior and cytokinic expression creating an environment permissive for early infection

G Castelli, F Bruno, L Saieva, R Alessandro… - Experimental …, 2019 - Elsevier
In recent years, several studies demonstrated the role of exosomes in intercellular
communications, several Leishmania species belonging to subgenera Leishmania and …

Leishmania infantum Induces Mild Unfolded Protein Response in Infected Macrophages

L Galluzzi, A Diotallevi, M De Santi, M Ceccarelli… - PloS one, 2016 - journals.plos.org
The Leishmaniases are a group of parasitic diseases caused by protozoa of the Leishmania
genus affecting both humans and other vertebrates. Leishmania is an intracellular pathogen …

Cultivation of Protozoa Parasites In Vitro: Growth Potential in Conventional Culture Media versus RPMI-PY Medium

G Castelli, E Oliveri, V Valenza, S Giardina… - Veterinary …, 2023 - mdpi.com
Simple Summary Many different media have been used to grow protozoa parasites, which
can be classified into two main categories: biphasic and liquid. All major biphasic media …

Bioactivity of Phycocolloids against the Mediterranean Protozoan Leishmania infantum: An Inceptive Study

S Armeli Minicante, S Michelet, F Bruno, G Castelli… - Sustainability, 2016 - mdpi.com
Sulfated polysaccharides from marine macroalgae have been shown to possess a variety of
biological activities against fungi, bacteria, viruses, and protozoa. In this study, the in vitro …

Cytotoxic Screening and In Vitro Evaluation of Pentadecane Against Leishmania infantum Promastigotes and Amastigotes

F Bruno, G Castelli, A Migliazzo… - Journal of …, 2015 - meridian.allenpress.com
Pentadecane is an organic compound that is made up of primarily carbon and hydrogen
atoms. Pentadecane is a floral volatile found in many different plants and essential oils and …

Meglumine Antimoniate‐Loaded Aqueous‐Core PLA Nanocapsules: Old Drug, New Formulation against Leishmania‐Related Diseases

D Cosco, F Bruno, G Castelli, R Puleio… - Macromolecular …, 2021 - Wiley Online Library
Leishmaniasis is a human and animal disease endemic in tropical and subtropical areas
treated by means of pentavalent antimony as first‐line approach. Unfortunately, the …