Nicotiana benthamiana: Its History and Future as a Model for Plant–Pathogen Interactions

MM Goodin, D Zaitlin, RA Naidu… - Molecular plant …, 2008 - Am Phytopath Society
Nicotiana benthamiana is the most widely used experimental host in plant virology, due
mainly to the large number of diverse plant viruses that can successfully infect it …

Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay

O Söderberg, KJ Leuchowius, M Gullberg, M Jarvius… - Methods, 2008 - Elsevier
The activity of proteins is typically regulated by secondary modifications and by interactions
with other partners, resulting in the formation of protein complexes whose functions depend …

Autophagic degradation of the 26S proteasome is mediated by the dual ATG8/ubiquitin receptor RPN10 in Arabidopsis

RS Marshall, F Li, DC Gemperline, AJ Book… - Molecular cell, 2015 - cell.com
Autophagic turnover of intracellular constituents is critical for cellular housekeeping, nutrient
recycling, and various aspects of growth and development in eukaryotes. Here we show that …

SAUR Inhibition of PP2C-D Phosphatases Activates Plasma Membrane H+-ATPases to Promote Cell Expansion in Arabidopsis

AK Spartz, H Ren, MY Park, KN Grandt, SH Lee… - The Plant …, 2014 - academic.oup.com
The plant hormone auxin promotes cell expansion. Forty years ago, the acid growth theory
was proposed, whereby auxin promotes proton efflux to acidify the apoplast and facilitate the …

Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta

R Waadt, LK Schmidt, M Lohse, K Hashimoto… - The Plant …, 2008 - Wiley Online Library
The specificity of intracellular signaling and developmental patterning in biological systems
relies on selective interactions between different proteins in specific cellular compartments …

Transient protein–protein interactions

SE Acuner Ozbabacan, HB Engin… - … , Design & Selection, 2011 - academic.oup.com
Transient complexes are crucial for diverse biological processes such as biochemical
pathways and signaling cascades in the cell. Here, we give an overview of the transient …

Transient expression in Nicotiana benthamiana fluorescent marker lines provides enhanced definition of protein localization, movement and interactions in planta

K Martin, K Kopperud, R Chakrabarty… - The Plant …, 2009 - Wiley Online Library
Here, we report on the construction of a novel series of Gateway‐compatible plant
transformation vectors containing genes encoding autofluorescent proteins, including …

Diversity in Genetic In Vivo Methods for Protein-Protein Interaction Studies: from the Yeast Two-Hybrid System to the Mammalian Split-Luciferase System

B Stynen, H Tournu, J Tavernier… - … and molecular biology …, 2012 - Am Soc Microbiol
The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries

BT DeVree, LM Steiner, S Głazowska, F Ruhnow… - Biotechnology for …, 2021 - Springer
Plant cell wall-derived biomass serves as a renewable source of energy and materials with
increasing importance. The cell walls are biomacromolecular assemblies defined by a fine …

Reconstitution of CO2 Regulation of SLAC1 Anion Channel and Function of CO2-Permeable PIP2;1 Aquaporin as CARBONIC ANHYDRASE4 Interactor

C Wang, H Hu, X Qin, B Zeise, D Xu, WJ Rappel… - The Plant …, 2016 - academic.oup.com
Dark respiration causes an increase in leaf CO2 concentration (Ci), and the continuing
increases in atmospheric [CO2] further increases Ci. Elevated leaf CO2 concentration …