[HTML][HTML] A critical review of bottom-up proteomics: the good, the bad, and the future of this field

EJ Dupree, M Jayathirtha, H Yorkey, M Mihasan… - Proteomes, 2020 - mdpi.com
Proteomics is the field of study that includes the analysis of proteins, from either a basic
science prospective or a clinical one. Proteins can be investigated for their abundance …

Transport phenomena in nanofluidics

RB Schoch, J Han, P Renaud - Reviews of modern physics, 2008 - APS
The transport of fluid in and around nanometer-sized objects with at least one characteristic
dimension below 100 nm enables the occurrence of phenomena that are impossible at …

[HTML][HTML] Proteomics: challenges, techniques and possibilities to overcome biological sample complexity

K Chandramouli, PY Qian - Human genomics and proteomics: HGP, 2009 - ncbi.nlm.nih.gov
Proteomics is the large-scale study of the structure and function of proteins in complex
biological sample. Such an approach has the potential value to understand the complex …

High-pH reversed-phase chromatography with fraction concatenation for 2D proteomic analysis

F Yang, Y Shen, DG Camp… - Expert review of proteomics, 2012 - Taylor & Francis
Orthogonal high-resolution separations are critical for attaining improved analytical dynamic
range and protein coverage in proteomic measurements. High-pH reversed-phase liquid …

Functional Proteomics of Arabidopsis thaliana Guard Cells Uncovers New Stomatal Signaling Pathways

Z Zhao, W Zhang, BA Stanley, SM Assmann - The Plant Cell, 2008 - academic.oup.com
We isolated a total of 3× 108 guard cell protoplasts from 22,000 Arabidopsis thaliana plants
and identified 1734 unique proteins using three complementary proteomic methods: protein …

[HTML][HTML] Middle-down approach: a choice to sequence and characterize proteins/proteomes by mass spectrometry

PB Pandeswari, V Sabareesh - RSC advances, 2019 - pubs.rsc.org
Owing to rapid growth in the elucidation of genome sequences of various organisms,
deducing proteome sequences has become imperative, in order to have an improved …

[HTML][HTML] Plant microbe interactions in post genomic era: perspectives and applications

J Imam, PK Singh, P Shukla - Frontiers in microbiology, 2016 - frontiersin.org
Deciphering plant–microbe interactions is a promising aspect to understand the benefits and
the pathogenic effect of microbes and crop improvement. The advancement in sequencing …

Organic− inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity

J Ma, Z Liang, X Qiao, Q Deng, D Tao, L Zhang… - Analytical …, 2008 - ACS Publications
A novel kind of immobilized trypsin reactor based on organic− inorganic hybrid silica
monoliths has been developed. With the presence of cetyltrimethyl ammonium bromide …

Practical implementation of 2D HPLC scheme with accurate peptide retention prediction in both dimensions for high-throughput bottom-up proteomics

RC Dwivedi, V Spicer, M Harder, M Antonovici… - Analytical …, 2008 - ACS Publications
We describe the practical implementation of a new RP (pH 10− pH 2) 2D HPLC− ESI/MS
scheme for large-scale bottom-up analysis in proteomics. When compared to the common …

Proteomic research in bivalves: Towards the identification of molecular markers of aquatic pollution

A Campos, S Tedesco, V Vasconcelos, S Cristobal - Journal of Proteomics, 2012 - Elsevier
Biomonitoring of aquatic environment and assessment of ecosystem health play essential
roles in the development of effective strategies for the protection of the environment, human …