[HTML][HTML] Observation and quantification of the morphological effect of trypan blue rupturing dead or dying cells
LLY Chan, WL Rice, J Qiu - PLoS One, 2020 - journals.plos.org
Trypan blue has long been the gold standard for staining dead cell to determine cell viability.
The dye is excluded from membrane-intact live cells, but can enter and concentrate in …
The dye is excluded from membrane-intact live cells, but can enter and concentrate in …
Morphological observation and analysis using automated image cytometry for the comparison of trypan blue and fluorescence-based viability detection method
LLY Chan, D Kuksin, DJ Laverty, S Saldi, J Qiu - Cytotechnology, 2015 - Springer
The ability to accurately determine cell viability is essential to performing a well-controlled
biological experiment. Typical experiments range from standard cell culturing to advanced …
biological experiment. Typical experiments range from standard cell culturing to advanced …
[HTML][HTML] Virtual global transplant laboratory standard operating procedures for blood collection, PBMC isolation, and storage
LE Higdon, K Lee, Q Tang, JS Maltzman - Transplantation direct, 2016 - journals.lww.com
Research on human immune responses frequently involves the use of peripheral blood
mononuclear cells (PBMC) immediately, or at significantly delayed timepoints, after …
mononuclear cells (PBMC) immediately, or at significantly delayed timepoints, after …
A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry
LLY Chan, T Smith, KA Kumph, D Kuksin, S Kessel… - Cytotechnology, 2016 - Springer
To ensure cell-based assays are performed properly, both cell concentration and viability
have to be determined so that the data can be normalized to generate meaningful and …
have to be determined so that the data can be normalized to generate meaningful and …
[HTML][HTML] An optimized workflow for single-cell transcriptomics and repertoire profiling of purified lymphocytes from clinical samples
R Hanamsagar, T Reizis, M Chamberlain, R Marcus… - Scientific reports, 2020 - nature.com
Establishing clinically relevant single-cell (SC) transcriptomic workflows from cryopreserved
tissue is essential to move this emerging immune monitoring technology from the bench to …
tissue is essential to move this emerging immune monitoring technology from the bench to …
[HTML][HTML] Sde-yolo: A novel method for blood cell detection
This paper proposes an improved target detection algorithm, SDE-YOLO, based on the
YOLOv5s framework, to address the low detection accuracy, misdetection, and leakage in …
YOLOv5s framework, to address the low detection accuracy, misdetection, and leakage in …
Quantitation of intracellular triphosphate metabolites of antiretroviral agents in peripheral blood mononuclear cells (PBMCs) and corresponding cell count …
D Xiao, KHJ Ling, J Custodio, SR Majeed… - Expert Opinion on …, 2018 - Taylor & Francis
Introduction: Peripheral blood mononuclear cells (PBMCs) are a critical component of the
immune system and the target cells for human immunodeficiency virus, type 1 (HIV-1) …
immune system and the target cells for human immunodeficiency virus, type 1 (HIV-1) …
Label-free, non-invasive, and repeatable cell viability bioassay using dynamic full-field optical coherence microscopy and supervised machine learning
We present a novel method that can assay cellular viability in real-time using supervised
machine learning and intracellular dynamic activity data that is acquired in a label-free, non …
machine learning and intracellular dynamic activity data that is acquired in a label-free, non …
The potential use of peripheral blood mononuclear cells as biomarkers for treatment response and outcome prediction in psychiatry: a systematic review
J Goossens, M Morrens, V Coppens - Molecular Diagnosis & Therapy, 2021 - Springer
Background Psychiatric disorders have a major impact on the global burden of disease
while therapeutic interventions remain insufficient to adequately treat a large number of …
while therapeutic interventions remain insufficient to adequately treat a large number of …
[PDF][PDF] Automatic detection and classification of malarial parasite
MI Razzak - International Journal of Biometrics and Bioinformatics …, 2015 - Citeseer
Recent advancement in genomic technologies has opened a new realm for early detection
of diseases that shows potential to overcome the drawbacks of manual detection …
of diseases that shows potential to overcome the drawbacks of manual detection …