[HTML][HTML] Observation and quantification of the morphological effect of trypan blue rupturing dead or dying cells

LLY Chan, WL Rice, J Qiu - PLoS One, 2020 - journals.plos.org
Trypan blue has long been the gold standard for staining dead cell to determine cell viability.
The dye is excluded from membrane-intact live cells, but can enter and concentrate in …

Morphological observation and analysis using automated image cytometry for the comparison of trypan blue and fluorescence-based viability detection method

LLY Chan, D Kuksin, DJ Laverty, S Saldi, J Qiu - Cytotechnology, 2015 - Springer
The ability to accurately determine cell viability is essential to performing a well-controlled
biological experiment. Typical experiments range from standard cell culturing to advanced …

[HTML][HTML] Virtual global transplant laboratory standard operating procedures for blood collection, PBMC isolation, and storage

LE Higdon, K Lee, Q Tang, JS Maltzman - Transplantation direct, 2016 - journals.lww.com
Research on human immune responses frequently involves the use of peripheral blood
mononuclear cells (PBMC) immediately, or at significantly delayed timepoints, after …

A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry

LLY Chan, T Smith, KA Kumph, D Kuksin, S Kessel… - Cytotechnology, 2016 - Springer
To ensure cell-based assays are performed properly, both cell concentration and viability
have to be determined so that the data can be normalized to generate meaningful and …

[HTML][HTML] An optimized workflow for single-cell transcriptomics and repertoire profiling of purified lymphocytes from clinical samples

R Hanamsagar, T Reizis, M Chamberlain, R Marcus… - Scientific reports, 2020 - nature.com
Establishing clinically relevant single-cell (SC) transcriptomic workflows from cryopreserved
tissue is essential to move this emerging immune monitoring technology from the bench to …

[HTML][HTML] Sde-yolo: A novel method for blood cell detection

Y Wu, D Gao, Y Fang, X Xu, H Gao, Z Ju - Biomimetics, 2023 - mdpi.com
This paper proposes an improved target detection algorithm, SDE-YOLO, based on the
YOLOv5s framework, to address the low detection accuracy, misdetection, and leakage in …

Quantitation of intracellular triphosphate metabolites of antiretroviral agents in peripheral blood mononuclear cells (PBMCs) and corresponding cell count …

D Xiao, KHJ Ling, J Custodio, SR Majeed… - Expert Opinion on …, 2018 - Taylor & Francis
Introduction: Peripheral blood mononuclear cells (PBMCs) are a critical component of the
immune system and the target cells for human immunodeficiency virus, type 1 (HIV-1) …

Label-free, non-invasive, and repeatable cell viability bioassay using dynamic full-field optical coherence microscopy and supervised machine learning

S Park, V Veluvolu, WS Martin, T Nguyen… - Biomedical Optics …, 2022 - opg.optica.org
We present a novel method that can assay cellular viability in real-time using supervised
machine learning and intracellular dynamic activity data that is acquired in a label-free, non …

The potential use of peripheral blood mononuclear cells as biomarkers for treatment response and outcome prediction in psychiatry: a systematic review

J Goossens, M Morrens, V Coppens - Molecular Diagnosis & Therapy, 2021 - Springer
Background Psychiatric disorders have a major impact on the global burden of disease
while therapeutic interventions remain insufficient to adequately treat a large number of …

[PDF][PDF] Automatic detection and classification of malarial parasite

MI Razzak - International Journal of Biometrics and Bioinformatics …, 2015 - Citeseer
Recent advancement in genomic technologies has opened a new realm for early detection
of diseases that shows potential to overcome the drawbacks of manual detection …