Assay methods for in vitro and in vivo anti-Babesia drug efficacy testing: Current progress, outlook, and challenges

MA Rizk, SAES El-Sayed, M Nassif, J Mosqueda… - Veterinary …, 2020 - Elsevier
Absence of an effective high-throughput drug-screening system for Babesia parasites is
considered one of the main causes for the presence of a wide gap in the treatment of animal …

Advances in understanding red blood cell modifications by Babesia

H Hakimi, J Yamagishi, S Kawazu, M Asada - PLoS Pathogens, 2022 - journals.plos.org
Babesia are tick-borne protozoan parasites that can infect livestock, pets, wildlife animals,
and humans. In the mammalian host, they invade and multiply within red blood cells (RBCs) …

Establishment of a stable transfection and gene targeting system in Babesia divergens

EFG Cubillos, P Snebergerova, S Borsodi… - Frontiers in Cellular …, 2023 - frontiersin.org
Babesia divergens is an emerging tick-borne pathogen considered as the principal
causative agent of bovine babesiosis in Europe with a notable zoonotic risk to human …

Whole-genome assembly of Babesia ovata and comparative genomics between closely related pathogens

J Yamagishi, M Asada, H Hakimi, TQ Tanaka… - BMC genomics, 2017 - Springer
Background Babesia ovata, belonging to the phylum Apicomplexa, is an infectious parasite
of bovids. It is not associated with the manifestation of severe symptoms, in contrast to other …

Establishment of a Transient and Stable Transfection System for Babesia duncani Using a Homologous Recombination Strategy

S Wang, D Li, F Chen, W Jiang, W Luo… - Frontiers in Cellular …, 2022 - frontiersin.org
Genetic modification provides an invaluable molecular tool to dissect the biology and
pathogenesis of pathogens. However, no report is available about the genetic modification …

A framework for signaling throughout the life cycle of Babesia species

B Elsworth, MT Duraisingh - Molecular microbiology, 2021 - Wiley Online Library
Babesia species are tick‐borne intracellular parasites that infect the red blood cells of their
mammalian host, leading to severe or fatal disease. Babesia spp. infect a wide range of …

Babesia ovata: Taxonomy, phylogeny and epidemiology

T Sivakumar, I Igarashi, N Yokoyama - Veterinary parasitology, 2016 - Elsevier
Babesia ovata, which is transmitted by Haemaphysalis longicornis, is an intraerythrocytic
protozoan parasite of cattle. Based on its morphology, B. ovata is classified as a large-type …

Genome Editing of Babesia bovis Using the CRISPR/Cas9 System

H Hakimi, T Ishizaki, Y Kegawa, O Kaneko, S Kawazu… - MSphere, 2019 - Am Soc Microbiol
Babesia bovis, the most virulent causative agent of bovine babesiosis, is prevalent in
tropical and subtropical regions of the world. Although the whole-genome sequence was …

Establishment of a novel tick-Babesia experimental infection model

H Maeda, T Hatta, MA Alim, D Tsubokawa, F Mikami… - Scientific reports, 2016 - nature.com
Ticks are potent vectors of many deadly human and animal pathogens. Tick-borne
babesiosis is a well-recognized malaria-like disease that occurs worldwide and recently has …

High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis

J Wang, A Liu, S Zhang, S Gao, M Rashid, Y Li, J Liu… - Parasites & vectors, 2019 - Springer
Background Bovine babesiosis is caused by protozoan parasites of the genus Babesia and
presents a wide spectrum of clinical manifestations. Disease severity depends on the type of …