Mycobiome diversity: high-throughput sequencing and identification of fungi

RH Nilsson, S Anslan, M Bahram… - Nature Reviews …, 2019 - nature.com
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …

Fungal diversity revisited: 2.2 to 3.8 million species

DL Hawksworth, R Lücking - Microbiology spectrum, 2017 - Am Soc Microbiol
The question of how many species of Fungi there are has occasioned much speculation,
with figures mostly posited from around half a million to 10 million, and in one extreme case …

Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

R Lücking, MC Aime, B Robbertse, AN Miller… - IMA fungus, 2020 - Springer
True fungi (Fungi) and fungus-like organisms (eg Mycetozoa, Oomycota) constitute the
second largest group of organisms based on global richness estimates, with around 3 …

Fungal identification using molecular tools: a primer for the natural products research community

HA Raja, AN Miller, CJ Pearce… - Journal of natural …, 2017 - ACS Publications
Fungi are morphologically, ecologically, metabolically, and phylogenetically diverse. They
are known to produce numerous bioactive molecules, which makes them very useful for …

Fungi in mycelium-based composites: usage and recommendations

M Sydor, G Cofta, B Doczekalska, A Bonenberg - Materials, 2022 - mdpi.com
Mycelium-Based Composites (MBCs) are innovative engineering materials made from
lignocellulosic by-products bonded with fungal mycelium. While some performance …

Improved software detection and extraction of ITS1 and ITS 2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing …

J Bengtsson‐Palme, M Ryberg… - Methods in ecology …, 2013 - Wiley Online Library
The nuclear ribosomal internal transcribed spacer (ITS) region is the primary choice for
molecular identification of fungi. Its two highly variable spacers (ITS 1 and ITS 2) are usually …

Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi

CL Schoch, KA Seifert, S Huhndorf… - Proceedings of the …, 2012 - National Acad Sciences
Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest
kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the …

One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes

JB Stielow, CA Levesque, KA Seifert… - … and Evolution of …, 2015 - ingentaconnect.com
The aim of this study was to assess potential candidate gene regions and corresponding
universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS …

New primers to amplify the fungal ITS2 region–evaluation by 454-sequencing of artificial and natural communities

K Ihrmark, ITM Bödeker, K Cruz-Martinez… - FEMS microbiology …, 2012 - academic.oup.com
With recent methodological advances, molecular markers are increasingly used for semi-
quantitative analyses of fungal communities. The aim to preserve quantitative relationships …

[PDF][PDF] Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi

L Tedersoo, S Anslan, M Bahram, S Põlme, T Riit… - …, 2015 - mycokeys.pensoft.net
Rapid development of high-throughput (HTS) molecular identification methods has
revolutionized our knowledge about taxonomic diversity and ecology of fungi. However …