The Mycobacterium tuberculosis complex (MTBC) consists of closely related species that
cause tuberculosis in both humans and animals. This illness, still today, remains to be one of …

Summary Polyphosphate kinase 1 (PPK1) helps bacteria to survive under stress. The ppk1
gene of Mycobacterium tuberculosis was overexpressed in Escherichia coli and …

Alternative ribosome subunit proteins are prevalent in the genomes of diverse bacterial
species, but their functional significance is controversial. Attempts to study microbial …

Using a component of the Escherichia coli protein degradation machinery, we have
established a system to regulate protein stability in mycobacteria. A protein tag derived from …

Mycobacterium tuberculosis is a serious worldwide health threat, killing almost 3 million
people per year. Other mycobacterial species, especially Mycobacterium avium, are …

Novel shuttle promoter-probe vectors replicating in Escherichia coli, Corynebacterium
glutamicum, and Rhodococcus erythropolis were constructed on the basis of the C …

With the increasing emergence of drug-resistant Mycobacterium tuberculosis strains, new
and effective antibiotics against tuberculosis (TB) are urgently needed. However, the high …

ABSTRACT A number of essential genes have been identified in mycobacteria, but methods
to study these genes have not been developed, leaving us unable to determine the function …

Reporter gene assays are widely used to study cellular signaling and transcriptional activity.
Few studies describe the use of reporter genes for studying cellular responses on complex …

Genotoxicity test systems that are based on bacteria display an important role in the
detection and assessment of DNA damaging chemicals. They belong to the basic line of test …