Phase transitions of associative biomacromolecules
Multivalent proteins and nucleic acids, collectively referred to as multivalent associative
biomacromolecules, provide the driving forces for the formation and compositional …
biomacromolecules, provide the driving forces for the formation and compositional …
The molecular basis for cellular function of intrinsically disordered protein regions
AS Holehouse, BB Kragelund - Nature Reviews Molecular Cell Biology, 2024 - nature.com
Intrinsically disordered protein regions exist in a collection of dynamic interconverting
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …
[HTML][HTML] A disordered region controls cBAF activity via condensation and partner recruitment
Intrinsically disordered regions (IDRs) represent a large percentage of overall nuclear
protein content. The prevailing dogma is that IDRs engage in non-specific interactions …
protein content. The prevailing dogma is that IDRs engage in non-specific interactions …
Engineering synthetic biomolecular condensates
The concept of phase-separation-mediated formation of biomolecular condensates provides
a new framework to understand cellular organization and cooperativity-dependent cellular …
a new framework to understand cellular organization and cooperativity-dependent cellular …
Visualizing the disordered nuclear transport machinery in situ
The approximately 120 MDa mammalian nuclear pore complex (NPC) acts as a gatekeeper
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …
RNAs undergo phase transitions with lower critical solution temperatures
GM Wadsworth, WJ Zahurancik, X Zeng, P Pullara… - Nature Chemistry, 2023 - nature.com
Co-phase separation of RNAs and RNA-binding proteins drives the biogenesis of
ribonucleoprotein granules. RNAs can also undergo phase transitions in the absence of …
ribonucleoprotein granules. RNAs can also undergo phase transitions in the absence of …
Macromolecular condensation organizes nucleolar sub-phases to set up a pH gradient
Nucleoli are multicomponent condensates defined by coexisting sub-phases. We identified
distinct intrinsically disordered regions (IDRs), including acidic (D/E) tracts and K-blocks …
distinct intrinsically disordered regions (IDRs), including acidic (D/E) tracts and K-blocks …
The molecular grammar of protein disorder guiding genome-binding locations
F Jonas, M Carmi, B Krupkin, J Steinberger… - Nucleic acids …, 2023 - academic.oup.com
Intrinsically disordered regions (IDRs) direct transcription factors (TFs) towards selected
genomic occurrences of their binding motif, as exemplified by budding yeast's Msn2 …
genomic occurrences of their binding motif, as exemplified by budding yeast's Msn2 …
HP1-driven phase separation recapitulates the thermodynamics and kinetics of heterochromatin condensate formation
MMC Tortora, LD Brennan… - Proceedings of the …, 2023 - National Acad Sciences
The spatial segregation of pericentromeric heterochromatin (PCH) into distinct, membrane-
less nuclear compartments involves the binding of Heterochromatin Protein 1 (HP1) to …
less nuclear compartments involves the binding of Heterochromatin Protein 1 (HP1) to …
The biophysics of disordered proteins from the point of view of single-molecule fluorescence spectroscopy
J Cubuk, MD Stuchell-Brereton… - Essays in …, 2022 - portlandpress.com
Intrinsically disordered proteins (IDPs) and regions (IDRs) have emerged as key players
across many biological functions and diseases. Differently from structured proteins …
across many biological functions and diseases. Differently from structured proteins …