To increase the temporal resolution and maximal imaging time of super-resolution (SR)
microscopy, we have developed a deconvolution algorithm for structured illumination …

Lipids play a vital role in the health and functioning of neurons and interest in the
physiological role of neuronal lipids is certainly increasing. One neuronal function in which …

Evoked exocytosis in excitable cells is fast and spatially confined and must be followed by
coupled endocytosis to enable sustained exocytosis while maintaining the balance of the …

In many non-excitable cells, the depletion of endoplasmic reticulum (ER) Ca2+ stores leads
to the dynamic formation of membrane contact sites (MCSs) between the ER and the plasma …

In subcellular light-sheet fluorescence microscopy (LSFM) of adherent cells, glass
substrates are advantageously rotated relative to the excitation and emission light paths to …

Abstract Endothelial exocytosis of Weibel–Palade body (WPB) is one of the first lines of
defence against vascular injury. However, the mechanisms that control WPB exocytosis in …

Heterogeneity of glucose-stimulated insulin secretion (GSIS) in pancreatic islets is
physiologically important but poorly understood. Here, we utilize mouse islets to determine …

The regulated secretory pathway begins with the formation of secretory granules by budding
from the Golgi apparatus and ends by their fusion with the plasma membrane leading to the …

Endoplasmic reticulum (ER)—plasma membrane (PM) contacts are dynamic structures with
important roles in the regulation of calcium (Ca2+) and lipid homeostasis. The extended …

Background Insulin is stored within large dense-core granules in pancreatic beta (β)-cells
and is released by Ca 2+-triggered exocytosis with increasing blood glucose levels …