Dynamic hydrogen bonds and hydrogen-bond networks are ubiquitous in proteins and
protein complexes. Functional roles that have been assigned to hydrogen-bond networks …

Neuronal activity causes use-dependent decline in protein function. However, it is unclear
how this is coupled to local quality control mechanisms. We show in Drosophila that the …

Hydrogen–deuterium exchange mass spectrometry (HDX-MS) is a powerful technique to
monitor protein intrinsic dynamics. The technique provides high-resolution information on …

Secretory preproteins of the Sec pathway are targeted post‐translationally and cross cellular
membranes through translocases. During cytoplasmic transit, mature domains remain non …

Membrane transporters and receptors often rely on conserved hydrogen bonds to assemble
transient paths for ion transfer or long-distance conformational couplings. For transporters …

Protein machines undergo conformational motions to interact with and manipulate polymeric
substrates. The Sec translocase promiscuously recognizes, becomes activated, and …

Single-molecule Förster resonance energy transfer (smFRET) enables the deconvolution of
various conformational substates of biomolecules. Over the past two decades, it has been …

Type III protein secretion is widespread in Gram-negative pathogens. It comprises the
injectisome with a surface-exposed needle and an inner membrane translocase. The …

Lipid membrane interfaces host reactions essential for the functioning of cells. The hydrogen-
bonding environment at the membrane interface is particularly important for binding of …

The bacterial Sec translocase transports unfolded proteins across membranes. In this issue
of Structure, Krishnamurthy et al.(2021) report a nexus of conformational dynamics in the …