Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …

Antibodies and antibody-derived molecules are continuously developed as both therapeutic
agents and key reagents for advanced diagnostic investigations. Their application in these …

The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …

The biopharmaceutical market is growing faster than ever, with two production systems
competing for market dominance: mammalian cells and microorganisms. In recent years …

The expression of correctly folded and functional heterologous proteins is important in many
biotechnological production processes, whether it is enzymes, biopharmaceuticals or …

Background Recombinant proteins are often engineered with an N-terminal signal peptide,
which facilitates their secretion to the oxidising environment of the periplasm (gram-negative …

Main reasons to produce recombinant proteins in the periplasm of E. coli rather than in its
cytoplasm are to-i-enable disulfide bond formation,-ii-facilitate protein isolation,-iii-control …

DegP is an oligomeric protein with dual protease and chaperone activity that regulates
protein homeostasis and virulence factor trafficking in the periplasm of gram-negative …

Analysis of intact proteins by native mass spectrometry has emerged as a powerful tool for
obtaining insight into subunit diversity, post-translational modifications, stoichiometry …

Background Physarum polycephalum is an unusual macroscopic myxomycete expressing a
large range of glycosyl hydrolases. Among them, enzymes from the GH18 family can …