[HTML][HTML] Recent developments in bioprocessing of recombinant proteins: expression hosts and process development
NK Tripathi, A Shrivastava - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …
humans worldwide. The production of therapeutic proteins for treating diseases at large …
[HTML][HTML] Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments
A Sandomenico, JP Sivaccumar, M Ruvo - International Journal of …, 2020 - mdpi.com
Antibodies and antibody-derived molecules are continuously developed as both therapeutic
agents and key reagents for advanced diagnostic investigations. Their application in these …
agents and key reagents for advanced diagnostic investigations. Their application in these …
[HTML][HTML] Factors involved in heterologous expression of proteins in E. coli host
M Pouresmaeil, S Azizi-Dargahlou - Archives of Microbiology, 2023 - Springer
The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …
biotechnology in the last century. These proteins are produced in the eukaryotic or …
[HTML][HTML] Microbial protein cell factories fight back?
LA Rettenbacher, K Arauzo-Aguilera, L Buscajoni… - Trends in …, 2022 - cell.com
The biopharmaceutical market is growing faster than ever, with two production systems
competing for market dominance: mammalian cells and microorganisms. In recent years …
competing for market dominance: mammalian cells and microorganisms. In recent years …
Folding of heterologous proteins in bacterial cell factories: Cellular mechanisms and engineering strategies
Y Rong, SI Jensen, K Lindorff-Larsen… - Biotechnology Advances, 2023 - Elsevier
The expression of correctly folded and functional heterologous proteins is important in many
biotechnological production processes, whether it is enzymes, biopharmaceuticals or …
biotechnological production processes, whether it is enzymes, biopharmaceuticals or …
[HTML][HTML] Increased production of periplasmic proteins in Escherichia coli by directed evolution of the translation initiation region
K Mirzadeh, PJ Shilling, R Elfageih, AJ Cumming… - Microbial cell …, 2020 - Springer
Background Recombinant proteins are often engineered with an N-terminal signal peptide,
which facilitates their secretion to the oxidising environment of the periplasm (gram-negative …
which facilitates their secretion to the oxidising environment of the periplasm (gram-negative …
[HTML][HTML] Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli
A Karyolaimos, JW de Gier - Frontiers in Bioengineering and …, 2021 - frontiersin.org
Main reasons to produce recombinant proteins in the periplasm of E. coli rather than in its
cytoplasm are to-i-enable disulfide bond formation,-ii-facilitate protein isolation,-iii-control …
cytoplasm are to-i-enable disulfide bond formation,-ii-facilitate protein isolation,-iii-control …
Competing stress-dependent oligomerization pathways regulate self-assembly of the periplasmic protease-chaperone DegP
DegP is an oligomeric protein with dual protease and chaperone activity that regulates
protein homeostasis and virulence factor trafficking in the periplasm of gram-negative …
protein homeostasis and virulence factor trafficking in the periplasm of gram-negative …
Mass spectrometry analysis of intact proteins from crude samples
Analysis of intact proteins by native mass spectrometry has emerged as a powerful tool for
obtaining insight into subunit diversity, post-translational modifications, stoichiometry …
obtaining insight into subunit diversity, post-translational modifications, stoichiometry …
[HTML][HTML] Characterization of chitinases from the GH18 gene family in the myxomycete Physarum polycephalum
S Renaud, A Dussutour, F Daboussi… - Biochimica et Biophysica …, 2023 - Elsevier
Background Physarum polycephalum is an unusual macroscopic myxomycete expressing a
large range of glycosyl hydrolases. Among them, enzymes from the GH18 family can …
large range of glycosyl hydrolases. Among them, enzymes from the GH18 family can …