Advances and challenges in biotechnological production of chondroitin sulfate and its oligosaccharides

W Zhang, R Xu, J Chen, H Xiong, Y Wang… - International journal of …, 2023 - Elsevier
Chondroitin sulfate (CS) is a member of glycosaminoglycans (GAGs) and has critical
physiological functions. CS is widely applied in medical and clinical fields. Currently, the …

[HTML][HTML] Design and biocatalytic applications of genetically fused multifunctional enzymes

DT Monterrey, I Ayuso-Fernández, I Oroz-Guinea… - Biotechnology …, 2022 - Elsevier
Fusion proteins, understood as those created by joining two or more genes that originally
encoded independent proteins, have numerous applications in biotechnology, from …

Characterization of interactions of dietary cholesterol with the murine and human gut microbiome

HH Le, MT Lee, KR Besler, JMC Comrie… - Nature …, 2022 - nature.com
Consumption of dietary lipids, such as cholesterol, modulates the gut microbiome with
consequences for host health through the production of microbiome-derived metabolites …

Engineering sulfonate group donor regeneration systems to boost biosynthesis of sulfated compounds

R Xu, W Zhang, X Xi, J Chen, Y Wang, G Du… - Nature …, 2023 - nature.com
Sulfonation as one of the most important modification reactions in nature is essential for
many biological macromolecules to function. Development of green sulfonate group donor …

Current state on the enzymatic synthesis of glycosaminoglycans

J Gottschalk, L Elling - Current opinion in chemical biology, 2021 - Elsevier
Glycosaminoglycans (GAGs) are linear anionic polysaccharides, and most of them show a
specific sulfation pattern. GAGs have been studied for decades, and still, new biological …

[HTML][HTML] Heterologous production of chondroitin

MR Couto, JL Rodrigues, LR Rodrigues - Biotechnology Reports, 2022 - Elsevier
Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a
popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources …

Closed-loop system driven by ADP phosphorylation from pyrophosphate affords equimolar transformation of ATP to 3′-phosphoadenosine-5′-phosphosulfate

R Xu, Y Wang, H Huang, X Jin, J Li, G Du, Z Kang - ACS Catalysis, 2021 - ACS Publications
3′-Phosphoadenosine-5′-phosphosulfate (PAPS) is a universal sulfate group donor for
all biological sulfation reactions in living organisms. Ambitions to biomanufacture sulfate …

Metabolic engineering of Escherichia coli for the production of an antifouling agent zosteric acid

P Zhang, J Gao, H Zhang, Y Wang, Z Liu, SY Lee… - Metabolic …, 2023 - Elsevier
Zosteric acid (ZA) is a Zostera species-derived, sulfated phenolic acid compound with
antifouling activity and has gained much attention due to its nontoxic and biodegradable …

From steroid and drug metabolism to glycobiology, using sulfotransferase structures to understand and tailor function

LC Pedersen, MJ Yi, LG Pedersen… - Drug Metabolism and …, 2022 - ASPET
Sulfotransferases are ubiquitous enzymes that transfer a sulfo group from the universal
cofactor donor 3′-phosphoadenosine 5′-phosphosulfate to a broad range of acceptor …

Biosynthetic production of anticoagulant heparin polysaccharides through metabolic and sulfotransferases engineering strategies

JQ Deng, Y Li, YJ Wang, YL Cao, SY Xin, XY Li… - Nature …, 2024 - nature.com
Heparin is an important anticoagulant drug, and microbial heparin biosynthesis is a potential
alternative to animal-derived heparin production. However, effectively using heparin …