Current state of the art for enhancing urine biomarker discovery

M Harpole, J Davis, V Espina - Expert review of Proteomics, 2016 - Taylor & Francis
Introduction: Urine is a highly desirable biospecimen for biomarker analysis because it can
be collected recurrently by non-invasive techniques, in relatively large volumes. Urine …

Biomarker discovery in mass spectrometry‐based urinary proteomics

S Thomas, L Hao, WA Ricke, L Li - PROTEOMICS–Clinical …, 2016 - Wiley Online Library
Urinary proteomics has become one of the most attractive topics in disease biomarker
discovery. MS‐based proteomic analysis has advanced continuously and emerged as a …

Proteomic characterization of hempseed (Cannabis sativa L.)

G Aiello, E Fasoli, G Boschin, C Lammi, C Zanoni… - Journal of …, 2016 - Elsevier
This paper presents an investigation on hempseed proteome. The experimental approach,
based on combinatorial peptide ligand libraries (CPLLs), SDS-PAGE separation, nLC-ESI …

Assessment of the floral origin of honey via proteomic tools

F Di Girolamo, A D'Amato, PG Righetti - Journal of proteomics, 2012 - Elsevier
The honey from chestnut, acacia, sunflower, eucalyptus and orange was analysed for its
proteome content, in order to see if any plant proteins present would allow the proteo-typing …

[HTML][HTML] In-depth exploration of cerebrospinal fluid by combining peptide ligand library treatment and label-free protein quantification

E Mouton-Barbosa, F Roux-Dalvai, D Bouyssié… - Molecular & Cellular …, 2010 - ASBMB
Cerebrospinal fluid (CSF) is the biological fluid in closest contact with the brain and thus
contains proteins of neural cell origin. Hence, CSF is a biochemical window into the brain …

With or without you—Proteomics with or without major plasma/serum proteins

E Gianazza, I Miller, L Palazzolo, C Parravicini… - Journal of …, 2016 - Elsevier
The first sections of this review compile and discuss strategies and protocols for managing
plasma/serum as a source of biomarkers relevant to human disease. In many such cases …

Resolubilization of precipitated intact membrane proteins with cold formic acid for analysis by mass spectrometry

AA Doucette, DB Vieira, DJ Orton… - Journal of Proteome …, 2014 - ACS Publications
Protein precipitation in organic solvent is an effective strategy to deplete sodium dodecyl
sulfate (SDS) ahead of MS analysis. Here we evaluate the recovery of membrane and water …

Advances in purification and separation of posttranslationally modified proteins

M Černý, J Skalák, H Cerna, B Brzobohatý - Journal of Proteomics, 2013 - Elsevier
Posttranslational modifications (PTMs) of proteins represent fascinating extensions of the
dynamic complexity of living cells' proteomes. The results of enzymatically catalyzed or …

[HTML][HTML] An experimental workflow for enrichment of low abundant proteins from human serum for the discovery of serum biomarkers

M Sarihan, MGB Albayrak, M Kasap… - Journal of biological …, 2023 - ncbi.nlm.nih.gov
Serum contains proteins that possess important information about diseases and their
progression. Unfortunately, these proteins, which carry the information in the serum are in …

From hundreds to thousands: Widening the normal human Urinome (1)

L Santucci, G Candiano, A Petretto, M Bruschi… - Journal of …, 2015 - Elsevier
It is currently unknown how many proteins can be detected in urine. Improving the analytical
approach would increase their number and potentially strengthen their predictive potential in …