Recombinant protein production is a multibillion-dollar market. The development of a new
product begins with the choice of a production host. While one single perfect host for every …

Glycoproteins are major players in the mucus protective barrier in the gastrointestinal and
other mucosal surfaces. In particular the mucus glycoproteins, or mucins, are responsible for …

We performed bottom-up engineering of a synthetic pathway in Escherichia coli for the
production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to …

Glycosylation is one of the most abundant and an important post-translational modification of
proteins. Glycosylated proteins (glycoproteins) are involved in various cellular biological …

Almost all therapeutic proteins are glycosylated, with the carbohydrate component playing a
long-established, substantial role in the safety and pharmacokinetic properties of this …

Protein expression in Escherichia coli represents the most facile approach for the
preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the …

Humans have been increasingly recognized as being superorganisms, living in close
contact with a microbiota on all their mucosal surfaces. However, most studies on the human …

Protein glycosylation is widespread throughout all three domains of life. Bacterial protein N-
glycosylation and its application to engineering recombinant glycoproteins continue to be …

Protein glycosylation, the attachment of sugars to amino acid side chains, can endow
proteins with a wide variety of properties of great interest to the engineering biology …

Recombinant DNA (rDNA) technologies allow the production of a wide range of peptides,
proteins and metabolites from naturally non-producing cells. Since human insulin was the …