When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Time-correlated single photon counting (TCSPC) is based on the detection of single photons
of a periodic light signal, measurement of the detection time of the photons, and the build-up …

Changing the data representation from the classical time delay histogram to the phasor
representation provides a global view of the fluorescence decay at each pixel of an image …

Background The root system of higher plants originates from the activity of a root meristem,
which comprises a group of highly specialized and long-lasting stem cells. Their …

Fluorescence is widely used in optical devices, microscopy imaging, biology, medical
research and diagnosis. Improving fluorescence sensitivity, all the way to the limit of single …

In fluorescence microscopy, the fluorescence emission can be characterised not only by
intensity and position, but also by lifetime, polarization and wavelength. Fluorescence …

Extracellular matrices (ECMs) are central to the advent of multicellular life, and their
mechanical properties are modulated by and impinge on intracellular signaling pathways …

Fluorescence lifetime imaging (FLIM) is widely applied to obtain quantitative information
from fluorescence signals, particularly using Förster Resonant Energy Transfer (FRET) …

Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial
distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems …