Production of biofuels and biochemicals by in vitro synthetic biosystems: opportunities and challenges

YHP Zhang - Biotechnology advances, 2015 - Elsevier
The largest obstacle to the cost-competitive production of low-value and high-impact
biofuels and biochemicals (called biocommodities) is high production costs catalyzed by …

Protein engineering of enzymes for process applications

JM Woodley - Current opinion in chemical biology, 2013 - Elsevier
Scientific progress in the field of enzyme modification today enables the opportunity to tune
a given biocatalyst for a specific industrial application. Much work has been focused on …

Hierarchically engineered mesoporous metal-organic frameworks toward cell-free immobilized enzyme systems

P Li, Q Chen, TC Wang, NA Vermeulen, BL Mehdi… - Chem, 2018 - cell.com
Highly efficient cell-free enzymatic systems are typically difficult to achieve in traditional
immobilized enzyme systems because of the lack of optimal spatial control of enzyme …

Engineering highly functional thermostable proteins using ancestral sequence reconstruction

Y Gumulya, JM Baek, SJ Wun, RES Thomson… - Nature Catalysis, 2018 - nature.com
Commercial biocatalysis requires robust enzymes that can withstand elevated temperatures
and long incubations. Ancestral reconstruction has shown that pre-Cambrian enzymes were …

Thermodynamics-Driven Production of Value-Added d-Allulose from Inexpensive Starch by an In Vitro Enzymatic Synthetic Biosystem

Y Li, T Shi, P Han, C You - ACS Catalysis, 2021 - ACS Publications
d-Allulose, which can be used as a food additive or functional food, is an important low-
calorie functional rare sugar. The current commercial production of d-allulose is performed …

New biotechnology paradigm: cell-free biosystems for biomanufacturing

JA Rollin, TK Tam, YHP Zhang - Green chemistry, 2013 - pubs.rsc.org
Cost-efficient production of sustainably-derived biochemicals and biofuels is a critical goal of
modern biotechnology. The current predominant biotransformation method is mainly based …

New biorefineries and sustainable agriculture: Increased food, biofuels, and ecosystem security

HG Chen, YHP Zhang - Renewable and Sustainable Energy Reviews, 2015 - Elsevier
The sustainability revolution is the defining challenge of our time to meet increasing needs
in the energy− food− water nexus without compromising the ability of next generations. To …

High‐yield production of dihydrogen from xylose by using a synthetic enzyme cascade in a cell‐free system

JS Martín del Campo, J Rollin, S Myung… - Angewandte …, 2013 - Wiley Online Library
Approximately 50 million metric tons of dihydrogen are produced annually from
nonrenewable natural gas, petroleum, and coal.[1] H2 production from water remains …

Cell-free systems for biosynthesis: Towards a sustainable and economical approach

MW Ullah, S Manan, M Ul-Islam, WA Khattak… - Green …, 2023 - pubs.rsc.org
The advent of modern technologies has inspired biotechnologists to shift towards in vitro
approaches that utilize cell-free environments for the generation of biochemicals and …

What is vital (and not vital) to advance economically-competitive biofuels production

YHP Zhang - Process Biochemistry, 2011 - Elsevier
Since biofuels is a hot topic, many researchers new to this field are eager to propose
different solutions while they often seem not to have full understanding of the current status …