Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives

J Dumont, D Euwart, B Mei, S Estes… - Critical reviews in …, 2016 - Taylor & Francis
Biotherapeutic proteins represent a mainstay of treatment for a multitude of conditions, for
example, autoimmune disorders, hematologic disorders, hormonal dysregulation, cancers …

Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing

R O'Flaherty, A Bergin, E Flampouri, LM Mota… - Biotechnology …, 2020 - Elsevier
The manufacturing of recombinant protein is traditionally undertaken in mammalian cell
culture. Today, speed, cost and safety are the primary considerations for process …

[HTML][HTML] Therapeutic glycoprotein production in mammalian cells

ME Lalonde, Y Durocher - Journal of biotechnology, 2017 - Elsevier
Over the last years, the biopharmaceutical industry has significantly turned its biologics
production towards mammalian cell expression systems. The presence of glycosylation …

The “less-is-more” in therapeutic antibodies: Afucosylated anti-cancer antibodies with enhanced antibody-dependent cellular cytotoxicity

NA Pereira, KF Chan, PC Lin, Z Song - MAbs, 2018 - Taylor & Francis
Therapeutic monoclonal antibodies are the fastest growing class of biological therapeutics
for the treatment of various cancers and inflammatory disorders. In cancer immunotherapy …

Sialic acid O-acetylation patterns and glycosidic linkage type determination by ion mobility-mass spectrometry

GM Vos, KC Hooijschuur, Z Li, J Fjeldsted… - Nature …, 2023 - nature.com
O-acetylation is a common modification of sialic acids that has been implicated in a
multitude of biological and disease processes. A lack of analytical methods that can …

Genome-wide survey of recurrent HBV integration in hepatocellular carcinoma

WK Sung, H Zheng, S Li, R Chen, X Liu, Y Li, NP Lee… - Nature …, 2012 - nature.com
To survey hepatitis B virus (HBV) integration in liver cancer genomes, we conducted
massively parallel sequencing of 81 HBV-positive and 7 HBV-negative hepatocellular …

The art of CHO cell engineering: A comprehensive retrospect and future perspectives

S Fischer, R Handrick, K Otte - Biotechnology advances, 2015 - Elsevier
Chinese hamster ovary (CHO) cells represent the most frequently applied host cell system
for industrial manufacturing of recombinant protein therapeutics. CHO cells are capable of …

Control of mucin-type O-glycosylation: a classification of the polypeptide GalNAc-transferase gene family

EP Bennett, U Mandel, H Clausen, TA Gerken… - …, 2012 - academic.oup.com
Glycosylation of proteins is an essential process in all eukaryotes and a great diversity in
types of protein glycosylation exists in animals, plants and microorganisms. Mucin-type O …

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

JY Kim, YG Kim, GM Lee - Applied microbiology and biotechnology, 2012 - Springer
Recombinant Chinese hamster ovary cells (rCHO) cells have been the most commonly used
mammalian host for large-scale commercial production of therapeutic proteins. Recent …

[HTML][HTML] Genome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential

G Zhang, X Liu, Z Quan, S Cheng, X Xu, S Pan… - Nature …, 2012 - nature.com
Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food
and fodder crop in arid regions and has potential for use as a C 4 biofuel. It is a model …