The tRNA modification field has a rich literature covering biochemical analysis going back
more than 40 years, but many of the corresponding genes were only identified in the last …

Efforts to map the Escherichia coli interactome have identified several hundred
macromolecular complexes, but direct binary protein-protein interactions (PPIs) have not …

The study of protein function usually requires the use of a cloned version of the gene for
protein expression and functional assays. This strategy is particularly important when the …

Background Membrane proteins are the targets of 50% of drugs, although they only
represent 1% of total cellular proteins. The first major bottleneck on the route to their …

More than 20% of all protein domains are currently annotated as “domains of unknown
function”(DUFs). About 2,700 DUFs are found in bacteria compared with just over 1,500 in …

The YgjD/Kae1 family (COG0533) has been on the top‐10 list of universally conserved
proteins of unknown function for over 5 years. It has been linked to DNA maintenance in …

The YbeB (DUF143) family of uncharacterized proteins is encoded by almost all bacterial
and eukaryotic genomes but not archaea. While they have been shown to be associated …

Two-hybrid systems are one of the most popular, preferred, cost effective, and scalable in
vivo genetic approaches for screening protein–protein interactions. A number of variants of …

Helicobacter pylori infections cause gastric ulcers and play a major role in the development
of gastric cancer. In 2001, the first protein interactome was published for this species …

Candida albicans is an opportunistic fungal pathogen that is responsible for infections linked
to high mortality. Loss‐of‐function approaches, taking advantage of gene knockouts or …