Chemically defined media for yeast cultivation (CDMY) were developed to support fast
growth, experimental reproducibility, and quantitative analysis of growth rates and biomass …

Covering: up to 2022 The report provides a broad approach to deciphering the evolution of
coenzyme biosynthetic pathways. Here, these various pathways are analyzed with respect …

Pyridoxal-5′-phosphate (PLP) is introduced to a biomimetic indicator displacement assay
for simultaneous determination of the absolute configuration, enantiomeric composition and …

Novel biophysical tools allow the structural dynamics of proteins and the regulation of such
dynamics by binding partners to be explored in unprecedented detail. Although this has …

Thiamine pyrophosphate (TPP), an essential co‐factor for all species, is biosynthesised
through a metabolically expensive pathway regulated by TPP riboswitches in bacteria, fungi …

While the majority of symbiosis research is focused on bacteria, microbial eukaryotes play
important roles in the microbiota and as pathogens, especially the incredibly diverse Fungi …

In biotin biosynthesis, the conversion of pimeloyl intermediates to biotin is catalyzed by a
universal set of four enzymes: BioF, BioA, BioD and BioB. We found that the gene …

Thiamin (vitamin B1) is an essential micronutrient needed as a cofactor for many central
metabolic enzymes. Animals must have thiamin in their diet, whereas bacteria, fungi, and …

Thiamin is essential for plant growth but is short-lived in vivo and energetically very costly to
produce–a combination that makes thiamin biosynthesis a prime target for improvement by …

The yeast thiamin pyrimidine synthase THI5p catalyzes one of the most complex organic
rearrangements found in primary metabolism. In this reaction, the active site His66 and PLP …