Nuclear DNA amounts in angiosperms: targets, trends and tomorrow

MD Bennett, IJ Leitch - Annals of botany, 2011 - academic.oup.com
Abstract Background and Aims The amount of DNA in an unreplicated gametic chromosome
complement is known as the C-value and is a key biodiversity character of fundamental …

Reference standards for flow cytometric estimation of absolute nuclear DNA content in plants

EM Temsch, P Koutecký, T Urfus, P Šmarda… - Cytometry Part …, 2022 - Wiley Online Library
The estimation of nuclear DNA content has been by far the most popular application of flow
cytometry in plants. Because flow cytometry measures relative fluorescence intensities of …

Estimation of nuclear DNA content in plants using flow cytometry

J Doležel, J Greilhuber, J Suda - Nature protocols, 2007 - nature.com
Flow cytometry (FCM) using DNA-selective fluorochromes is now the prevailing method for
the measurement of nuclear DNA content in plants. Ease of sample preparation and high …

Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species

J Loureiro, E Rodriguez, J Doležel, C Santos - Annals of botany, 2007 - academic.oup.com
Abstract Background and Aims After the initial boom in the application of flow cytometry in
plant sciences in the late 1980s and early 1990s, which was accompanied by development …

The application of flow cytometry for estimating genome size, ploidy level endopolyploidy, and reproductive modes in plants

J Pellicer, RF Powell, IJ Leitch - Molecular plant taxonomy: methods and …, 2021 - Springer
Over the years, the amount of DNA in a nucleus (genome size) has been estimated using a
variety of methods, but increasingly, flow cytometry (FCM) has become the method of choice …

Application‐based guidelines for best practices in plant flow cytometry

E Sliwinska, J Loureiro, IJ Leitch, P Šmarda… - Cytometry Part …, 2022 - Wiley Online Library
Flow cytometry (FCM) is currently the most widely‐used method to establish nuclear DNA
content in plants. Since simple, 1‐3‐parameter, flow cytometers, which are sufficient for most …

Evolution of genome size and chromosome number in the carnivorous plant genus Genlisea (Lentibulariaceae), with a new estimate of the minimum genome size in …

A Fleischmann, TP Michael, F Rivadavia… - Annals of …, 2014 - academic.oup.com
Abstract Background and Aims Some species of Genlisea possess ultrasmall nuclear
genomes, the smallest known among angiosperms, and some have been found to have …

Applications of flow cytometry to evolutionary and population biology

P Kron, J Suda, BC Husband - Annu. Rev. Ecol. Evol. Syst., 2007 - annualreviews.org
Flow cytometry, a method of rapidly characterizing optical properties of cells and cell
components within individuals, populations, and communities, is advancing research in …

New reports of nuclear DNA content for 407 vascular plant taxa from the United States

C Bai, WS Alverson, A Follansbee… - Annals of botany, 2012 - academic.oup.com
Abstract Background and Aims The amount of DNA in an unreplicated haploid nuclear
genome (C-value) ranges over several orders of magnitude among plant species and …

[PDF][PDF] Naturalized plants have smaller genomes than their non-invading relatives: a flow cytometric analysis of the Czech alien flora

M Kubešová, L Moravcova, J Suda, V Jarošík, P Pyšek - Preslia, 2010 - researchgate.net
Genome size has been suggested as one of the traits associated with invasiveness of plant
species. To provide a quantitative insight into the role of this trait, we estimated nuclear DNA …