Thermostability engineering of industrial enzymes through structure modification

NG Nezhad, RNZRA Rahman, YM Normi… - Applied Microbiology …, 2022 - Springer
Thermostability is an essential requirement of enzymes in the industrial processes to
catalyze the reactions at high temperatures; thus, enzyme engineering through directed …

Thermostable cellulose saccharifying microbial enzymes: Characteristics, recent advances and biotechnological applications

A Dadwal, S Sharma, T Satyanarayana - International Journal of Biological …, 2021 - Elsevier
Cellulases play a promising role in the bioconversion of renewable lignocellulosic biomass
into fermentable sugars which are subsequently fermented to biofuels and other value …

Recent progress in key lignocellulosic enzymes: Enzyme discovery, molecular modifications, production, and enzymatic biomass saccharification

Y Li, W Song, X Han, Y Wang, S Rao, Q Zhang… - Bioresource …, 2022 - Elsevier
Lignocellulose, the most prevalent biomass on earth, can be enzymatically converted into
carbohydrates for bioethanol production and other uses. Among lignocellulosic enzymes …

Overview of strategies for developing high thermostability industrial enzymes: Discovery, mechanism, modification and challenges

H Wu, Q Chen, W Zhang, W Mu - Critical Reviews in Food Science …, 2023 - Taylor & Francis
Biocatalysts such as enzymes are environmentally friendly and have substrate specificity,
which are preferred in the production of various industrial products. However, the strict …

Coenzyme engineering of glucose-6-phosphate dehydrogenase on a nicotinamide-based biomimic and its application as a glucose biosensor

D Meng, M Liu, H Su, H Song, L Chen, Q Li, Y Liu… - ACS …, 2023 - ACS Publications
Nicotinamide adenine dinucleotide (NAD (P)+)-dependent oxidoreductases have been
widely employed as biocatalysts for numerous applications, such as in vitro …

Recent advances in structure‐based enzyme engineering for functional reconstruction

SY Xu, L Zhou, Y Xu, HY Hong, C Dai… - Biotechnology and …, 2023 - Wiley Online Library
Structural information can help engineer enzymes. Usually, specific amino acids in particular
regions are targeted for functional reconstruction to enhance the catalytic performance …

Comprehensive Understanding of Laboratory Evolution for Food Enzymes: From Design to Screening Innovations

J Wu, Z Wang, M Zeng, Z He, Q Chen… - Journal of Agricultural …, 2024 - ACS Publications
In the field of food processing, enzymes play a pivotal role in improving product quality and
flavor, and extending shelf life. However, the exposure of traditional food enzymes to high …

KnowVolution of a GH5 Cellulase from Penicillium verruculosum to Improve Thermal Stability for Biomass Degradation

F Contreras, MJ Thiele, S Pramanik… - ACS Sustainable …, 2020 - ACS Publications
Understanding the thermostability of cellulases is of high importance for their application in
lignocellulosic biomass degradation, feedstock, and pulp and paper production. Cellulases …

Hotspots and mechanisms of action of the thermostable framework of a microbial thermolipase

Y Fang, F Liu, Y Shi, T Yang, C Liang, Y Xin… - ACS Synthetic …, 2022 - ACS Publications
The lipase TrLipB from Thermomicrobium roseum is highly thermostable. However, its
thermostable skeleton and mechanism of action should be investigated for industrial …

[HTML][HTML] Engineering the thermostability of D-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient …

H Wu, M Yi, X Wu, Y Ding, M Pu, L Wen… - Synthetic and Systems …, 2023 - Elsevier
Abstract d-Mannose is an attractive functional sugar that exhibits many physiological
benefits on human health. The demand for low-calorie sugars and sweeteners in foods are …