Reversed-phase liquid chromatography of peptides for bottom-up proteomics: a tutorial

J Lenco, S Jadeja, DK Naplekov… - Journal of Proteome …, 2022 - ACS Publications
The performance of the current bottom-up liquid chromatography hyphenated with mass
spectrometry (LC-MS) analyses has undoubtedly been fueled by spectacular progress in …

Core-shell particles lead the way to renewing high-performance liquid chromatography

V González-Ruiz, AI Olives, MA Martín - TrAC Trends in Analytical …, 2015 - Elsevier
In this review, we present the latest highlights and trends in the use of core-shell particles as
stationary phases in columns for liquid chromatography (LC). These highly homogeneous …

LC–MS bioanalysis of intact proteins and peptides

L Kang, N Weng, W Jian - Biomedical chromatography, 2020 - Wiley Online Library
Bioanalysis assays that reliably quantify biotherapeutics and biomarkers in biological
samples play pivotal roles in drug discovery and development. Liquid chromatography …

Sol‐Gel and Porous Glass‐Based Silica Monoliths with Hierarchical Pore Structure for Solid‐Liquid Catalysis

D Enke, R Gläser, U Tallarek - Chemie Ingenieur Technik, 2016 - Wiley Online Library
This work draws attention to both prospects and challenges for silica monoliths in solid‐
liquid catalysis at ultrahigh efficiency. The fundamental advantages of silica monoliths as …

Computational investigation of longitudinal diffusion, eddy dispersion, and trans-particle mass transfer in bulk, random packings of core–shell particles with varied …

A Daneyko, D Hlushkou, V Baranau, S Khirevich… - … of Chromatography A, 2015 - Elsevier
In recent years, chromatographic columns packed with core–shell particles have been
widely used for efficient and fast separations at comparatively low operating pressure …

Simultaneous LC–MS determination of glucose regulatory peptides secreted by stem cell–derived islet organoids

C Olsen, C Wang, A Aizenshtadt, S Abadpour… - …, 2023 - Wiley Online Library
For studying stem cell–derived islet organoids (SC‐islets) in an organ‐on‐chip (OoC)
platform, we have developed a reversed‐phase liquid chromatography–tandem mass …

[HTML][HTML] Determination of insulin secretion from stem cell-derived islet organoids with liquid chromatography-tandem mass spectrometry

C Olsen, C Wang, S Abadpour, E Lundanes… - … of Chromatography B, 2023 - Elsevier
Organoids are laboratory-grown 3D organ models, mimicking human organs for eg drug
development and personalized therapy. Islet organoids (typically 100–200 µm), which can …

Speed-resolution properties of columns packed with new 4.6 μm Kinetex-C18 core–shell particles

F Gritti, G Guiochon - Journal of Chromatography A, 2013 - Elsevier
The achievable separation speed and resolution of columns packed with the new 4.6 μm
Kinetex particles were characterized by their permeability and their plate heights, measured …

New developments and possibilities of wide-pore superficially porous particle technology applied for the liquid chromatographic analysis of therapeutic proteins

B Bobály, JL Veuthey, D Guillarme, S Fekete - Journal of pharmaceutical …, 2018 - Elsevier
This review paper discusses the success of columns packed with superficially porous
particles (SPP) in liquid chromatography for the analysis of peptides and proteins. First, it …

Rapid development of core–shell column technology: accurate measurements of the intrinsic column efficiency of narrow-bore columns packed with 4.6 down to 1.3 …

F Gritti, G Guiochon - Journal of Chromatography A, 2014 - Elsevier
The intrinsic heights equivalent to a theoretical plate (HETPs) of 31 narrow-bore and wide-
bore columns packed with four different brands of core–shell particles were accurately …