One of the aims of modern microscopy is to quantify two-, three-, or even four-dimensional
phenomena in biology, medicine, and material sciences. The requirements imposed on …

The ribosome is a large multifunctional complex composed of both RNA and proteins.
Biophysical methods are yielding low-resolution structures of the overall architecture of …

Using the atomic structures of the large ribosomal subunit from Haloarcula marismortui and
its complexes with two substrate analogs, we establish that the ribosome is a ribozyme and …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

The small ribosomal subunit performs the decoding of genetic information during translation.
The structure of that from Thermus thermophilus shows that the decoding center, which …

1. Introduction 3082. Electron microscopy 3112.1 Specimen preparation 3112.2 The
electron microscope 3112.3 Acceleration voltage, defocus, and the electron gun 3122.4 …

Structures of 70 S ribosome complexes containing messenger RNA and transfer RNA
(tRNA), or tRNA analogs, have been solved by x-ray crystallography at up to 7.8 angstrom …

Recently, the atomic structures of the large ribosomal subunit from Haloarcula marismortui
and its complexes with substrates have been determined. These have provided exciting new …

After a general introduction to three-dimensional electron microscopy and particularly to
electron tomography (ET), the perspectives of applying ET to native (frozen–hydrated) …

We present a detailed analysis of the protein structures in the 30 S ribosomal subunit from
Thermus thermophilus, and their interactions with 16 S RNA based on a crystal structure at …