DNA mechanics and its biological impact

A Basu, DG Bobrovnikov, T Ha - Journal of molecular biology, 2021 - Elsevier
Almost all nucleoprotein interactions and DNA manipulation events involve mechanical
deformations of DNA. Extraordinary progresses in single-molecule, structural, and …

Adaptive homeostasis and the p53 isoform network

S Mehta, H Campbell, CJ Drummond, K Li, K Murray… - EMBO …, 2021 - embopress.org
All living organisms have developed processes to sense and address environmental
changes to maintain a stable internal state (homeostasis). When activated, the p53 tumour …

[HTML][HTML] Structural basis for Cas9 off-target activity

M Pacesa, CH Lin, A Cléry, A Saha, PR Arantes… - Cell, 2022 - cell.com
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …

Geometric deep learning of protein–DNA binding specificity

R Mitra, J Li, JM Sagendorf, Y Jiang, AS Cohen… - Nature …, 2024 - nature.com
Predicting protein–DNA binding specificity is a challenging yet essential task for
understanding gene regulation. Protein–DNA complexes usually exhibit binding to a …

RNA conformational propensities determine cellular activity

ML Ken, R Roy, A Geng, LR Ganser, A Manghrani… - Nature, 2023 - nature.com
Cellular processes are the product of interactions between biomolecules, which associate to
form biologically active complexes. These interactions are mediated by intermolecular …

Deciphering the Role of the Ser‐Phosphorylation Pattern on the DNA‐Binding Activity of Max Transcription Factor Using Chemical Protein Synthesis

RV Nithun, YM Yao, X Lin, S Habiballah… - Angewandte Chemie …, 2023 - Wiley Online Library
The chemical synthesis of site‐specifically modified transcription factors (TFs) is a powerful
method to investigate how post‐translational modifications (PTMs) influence TF‐DNA …

Predicting DNA structure using a deep learning method

J Li, TP Chiu, R Rohs - Nature Communications, 2024 - nature.com
Understanding the mechanisms of protein-DNA binding is critical in comprehending gene
regulation. Three-dimensional DNA structure, also described as DNA shape, plays a key …

High-throughput data and modeling reveal insights into the mechanisms of cooperative DNA-binding by transcription factor proteins

V Martin, F Zhuang, Y Zhang, K Pinheiro… - Nucleic Acids …, 2023 - academic.oup.com
Cooperative DNA-binding by transcription factor (TF) proteins is critical for eukaryotic gene
regulation. In the human genome, many regulatory regions contain TF-binding sites in close …

Physicochemical models of protein–DNA binding with standard and modified base pairs

TP Chiu, S Rao, R Rohs - Proceedings of the National …, 2023 - National Acad Sciences
DNA-binding proteins play important roles in various cellular processes, but the
mechanisms by which proteins recognize genomic target sites remain incompletely …

Single-particle combinatorial multiplexed liposome fusion mediated by DNA

MG Malle, PMG Löffler, SSR Bohr, MB Sletfjerding… - Nature Chemistry, 2022 - nature.com
Combinatorial high-throughput methodologies are central for both screening and discovery
in synthetic biochemistry and biomedical sciences. They are, however, often reliant on large …