G protein–coupled receptors (GPCRs) are a large family comprising> 800 signaling
receptors that regulate numerous cellular and physiologic responses. GPCRs have been …

Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

Notch has been implicated in human cancers and is a putative therapeutic target. However,
the regulation of Notch activation in the nucleus remains largely uncharacterized. Therefore …

Ubiquitin (Ub) chain formation by homologous to E6AP C-terminus (HECT)-family E3 ligases
regulates vast biology, yet the structural mechanisms remain unknown. We used chemistry …

Lysine (Lys) residues in proteins undergo a wide range of reversible post-translational
modifications (PTMs), which can regulate enzyme activities, chromatin structure, protein …

Ubiquitination plays a pivotal role in several cellular processes and is critical for protein
degradation and signaling. E3 ubiquitin ligases are the matchmakers in the ubiquitination …

Ubiquitination, the covalent attachment of ubiquitin to proteins, by E3 ligases of the HECT
(homologous to E6AP C terminus) family is critical in controlling diverse physiological …

Lipid homeostasis is essential for normal cellular functions and dysregulation of lipid
metabolism is highly correlated with human diseases including neurodegenerative …

Akt is a critical protein kinase that drives cancer proliferation, modulates metabolism, and is
activated by C-terminal phosphorylation. The current structural model for Akt activation by C …

Ubiquitin ligases (E3 enzymes) transfer ubiquitin from ubiquitin-conjugating (E2) enzymes to
target proteins. By determining the selection of target proteins, modification sites on those …