Two decades ago, theoretical model calculations played a truly minor role in understanding
mechanisms of redox-active metalloenzymes. The methods available to treat transition …

Highlights•CSIA bears great potential to evaluate micropollutant degradation in the
environment.•We review analytical challenges for CSIA of low concentration, polar …

Serum paraoxonase 1 (PON1) is a native lactonase capable of promiscuously hydrolyzing a
broad range of substrates, including organophosphates, esters, and carbonates …

This review is devoted to the stereochemistry of nucleophilic substitution reactions at
phosphorus. The study of the reactions of phosphoryl group transfer is important for …

Highlights•Enzyme promiscuity is widespread and important for the evolution of enzyme
function.•Alkaline phosphatases (APs) are a model system for understanding enzyme …

The re-emergence of chemical weapons as a global threat in hands of terrorist groups,
together with an increasing number of pesticides intoxications and environmental …

The occurrence of organophosphorus compounds, pesticides, and flame-retardants in
wastes is an emerging ecological problem. Bacterial phosphotriesterases are capable of …

In recent years, it has become increasingly clear that promiscuity plays a key role in the
evolution of new enzyme function. This finding has helped to elucidate fundamental aspects …

The enzymes that facilitate phosphate and sulfate hydrolysis are among the most proficient
natural catalysts known to date. Interestingly, a large number of these enzymes are …

Diverse organophosphate hydrolases have convergently evolved the ability to hydrolyse
man-made organophosphates. Thus, these enzymes are attractive model systems for …