Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine

Y Su, C Liu, H Fang, D Zhang - Microbial cell factories, 2020 - Springer
Due to its clear inherited backgrounds as well as simple and diverse genetic manipulation
systems, Bacillus subtilis is the key Gram-positive model bacterium for studies on physiology …

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

P Calero, PI Nikel - Microbial Biotechnology, 2019 - Wiley Online Library
The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …

Advances and prospects of Bacillus subtilis cellular factories: from rational design to industrial applications

Y Gu, X Xu, Y Wu, T Niu, Y Liu, J Li, G Du, L Liu - Metabolic engineering, 2018 - Elsevier
Bacillus subtilis is the most characterized gram-positive bacterium that has significant
attributes, such as growing well on cheap carbon sources, possessing clear inherited …

Tat-Independent Secretion of Polyethylene Terephthalate Hydrolase PETase in Bacillus subtilis 168 Mediated by Its Native Signal Peptide

X Huang, L Cao, Z Qin, S Li, W Kong… - Journal of agricultural …, 2018 - ACS Publications
Widespread utilization of polyethylene terephthalate (PET) has caused critical
environmental pollution. The enzymatic degradation of PET is a promising solution to this …

β‐Galactosidase: From its source and applications to its recombinant form

A Movahedpour, N Ahmadi… - Biotechnology and …, 2022 - Wiley Online Library
Carbohydrate‐active enzymes are a group of important enzymes playing a critical role in the
degradation and synthesis of carbohydrates. Glycosidases can hydrolyze glycosides into …

Engineering Bacillus for efficient production of heterologous protein: current progress, challenge and prospect

D Cai, Y Rao, Y Zhan, Q Wang… - Journal of applied …, 2019 - academic.oup.com
Bacillus species are widely recognized as good industrial platform strains, which can
produce a variety of valuable products including enzymes and functional proteins. Bacillus …

Bacterial excretion of cytoplasmic proteins (ECP): occurrence, mechanism, and function

P Ebner, F Götz - Trends in microbiology, 2019 - cell.com
The excretion of cytoplasmic and signal-peptide-less proteins (ECP) by microorganisms and
eukaryotes remains a fascinating topic. In principle, it appears to be a waste of energy …

Secretory expression of recombinant small laccase genes in Gram-positive bacteria

S Välimets, P Pedetti, LJ Virginia, MN Hoang… - Microbial Cell …, 2023 - Springer
Background Laccases are multicopper enzymes that oxidize a wide range of aromatic and
non-aromatic compounds in the presence of oxygen. The majority of industrially relevant …

CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis

S Yu, MA Price, Y Wang, Y Liu, Y Guo, X Ni… - ACS Synthetic …, 2020 - ACS Publications
Base editing technology based on clustered regularly interspaced short palindromic
repeats/associated protein 9 (CRISPR/Cas9) is a recent addition to the family of CRISPR …

The multifunctionality of expression systems in Bacillus subtilis: Emerging devices for the production of recombinant proteins

CC Souza, JM Guimarães, SS Pereira… - Experimental …, 2021 - journals.sagepub.com
Bacillus subtilis is a successful host for producing recombinant proteins. Its GRAS (generally
recognized as safe) status and its remarkable innate ability to absorb and incorporate …