RNA sequencing: the teenage years
Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …
transcriptome-wide analysis of differential gene expression and differential splicing of …
Beyond mass spectrometry, the next step in proteomics
Proteins can be the root cause of a disease, and they can be used to cure it. The need to
identify these critical actors was recognized early (1951) by Sanger; the first biopolymer …
identify these critical actors was recognized early (1951) by Sanger; the first biopolymer …
A split prime editor with untethered reverse transcriptase and circular RNA template
Delivery and optimization of prime editors (PEs) have been hampered by their large size
and complexity. Although split versions of genome-editing tools can reduce construct size …
and complexity. Although split versions of genome-editing tools can reduce construct size …
Engineered CRISPR prime editors with compact, untethered reverse transcriptases
J Grünewald, BR Miller, RN Szalay… - Nature …, 2023 - nature.com
The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase
(nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase …
(nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase …
High-resolution quantitative profiling of tRNA abundance and modification status in eukaryotes by mim-tRNAseq
A Behrens, G Rodschinka, DD Nedialkova - Molecular cell, 2021 - cell.com
Measurements of cellular tRNA abundance are hampered by pervasive blocks to cDNA
synthesis at modified nucleosides and the extensive similarity among tRNA genes. We …
synthesis at modified nucleosides and the extensive similarity among tRNA genes. We …
Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs
Y Xu, TF Zhu - Science, 2022 - science.org
To synthesize a chirally inverted ribosome with the goal of building mirror-image biology
systems requires the preparation of kilobase-long mirror-image ribosomal RNAs that make …
systems requires the preparation of kilobase-long mirror-image ribosomal RNAs that make …
Evolution of a reverse transcriptase to map N1-methyladenosine in human messenger RNA
Chemical modifications to messenger RNA are increasingly recognized as a critical
regulatory layer in the flow of genetic information, but quantitative tools to monitor RNA …
regulatory layer in the flow of genetic information, but quantitative tools to monitor RNA …
A rapid RIG-I signaling relay mediates efficient antiviral response
RIG-I is essential for host defense against viral pathogens, as it triggers the release of type I
interferons upon encounter with viral RNA molecules. In this study, we show that RIG-I is …
interferons upon encounter with viral RNA molecules. In this study, we show that RIG-I is …
Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria
The ability to determine full-length nucleotide composition of individual RNA molecules is
essential for understanding the architecture and function of a transcriptome. However …
essential for understanding the architecture and function of a transcriptome. However …
Cis-mediated interactions of the SARS-CoV-2 frameshift RNA alter its conformations and affect function
L Pekarek, MM Zimmer, AS Gribling-Burrer… - Nucleic Acids …, 2023 - academic.oup.com
The RNA genome of SARS-CoV-2 contains a frameshift stimulatory element (FSE) that
allows access to an alternative reading frame through− 1 programmed ribosomal …
allows access to an alternative reading frame through− 1 programmed ribosomal …