[HTML][HTML] Long-term preservation of germ cells and gonadal tissues at ambient temperatures

P Comizzoli, X He, PC Lee - Reproduction and Fertility, 2022 - raf.bioscientifica.com
Objective To present an overview of different approaches and recent advances for long-term
preservation of germ cells and gonadal tissues at ambient temperatures. Methods Review of …

Shipping and logistics considerations for regenerative medicine therapies

T Criswell, C Swart, J Stoudemire… - Stem Cells …, 2022 - academic.oup.com
Advances in regenerative medicine manufacturing continue to be a priority for achieving the
full commercial potential of important breakthrough therapies. Equally important will be the …

Cryoprotection in Human Mesenchymal Stromal/Stem Cells: Synergistic Impact of Urea and Glucose

M Kardorff, HC Mahler, J Huwyler, D Jere… - Journal of Pharmaceutical …, 2023 - Elsevier
Standard freezing protocols of clinically relevant cell lines commonly employ agents such as
fetal bovine serum and dimethyl sulfoxide, which are a potential concern from both a …

Cell Therapy Drug Product Development: Technical Considerations and Challenges

L Weng - Journal of Pharmaceutical Sciences, 2023 - Elsevier
Cell therapy uses living cells as a drug to treat diseases. To develop a cell therapy drug
product (DP), cryopreservation plays a central role in extending the shelf life of these living …

Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

P Comizzoli, O Amelkina, PC Lee - Molecular Reproduction and …, 2022 - Wiley Online Library
Long‐term preservation of sperm, oocytes, and gonadal tissues at ambient temperatures
has the potential to lower the costs and simplify biobanking in human reproductive medicine …

Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations

R Brogna, J Fan, H Sieme, WF Wolkers, H Oldenhof - Scientific reports, 2021 - nature.com
Even though dried sperm is not viable, it can be used for fertilization as long as its chromatin
remains intact. In this study, we investigated drying-and temperature-induced conformational …

Microwave-assisted dehydration, long-term storage at non-freezing temperatures, and rehydration of cat germinal vesicles

PC Lee, P Comizzoli - Biology of Reproduction, 2024 - academic.oup.com
Germinal vesicles are alternative targets for female fertility preservation due to their
availability and high resilience against non-physiological conditions. Preserved germinal …

The Group 3 LEA proteins of Artemia franciscana for cryopreservation

L Li, X Zhou, Z Chen, Y Cao, G Zhao - Cryobiology, 2022 - Elsevier
Late embryogenesis-abundant (LEA) proteins are protective proteins that are enriched in the
late stage of seed embryo development. LEA proteins play an important role in resisting …

Enabling Clonal Analyses of Yeast in Outer Space by Encapsulation and Desiccation in Hollow Microparticles

S Ng, C Williamson, M van Zee, D Di Carlo… - Life, 2022 - mdpi.com
Studying microbes at the single-cell level in space can accelerate human space exploration
both via the development of novel biotechnologies and via the understanding of cellular …

Marine Biobank: From Protection of Genetic Resources to Biomedical Entrepreneurship

T Zendehboudi, AR Afshar, A Khoradmehr… - Iranian South Medical …, 2021 - ismj.bpums.ac.ir
Background Marine biological resources and their creatures are one of the major parts of
marine biomass. Marine biological resources are important for regular genetic research and …