Advanced point‐of‐care testing technologies for human acute respiratory virus detection
The ever‐growing global threats to human life caused by the human acute respiratory virus
(RV) infections have cost billions of lives, created a significant economic burden, and …
(RV) infections have cost billions of lives, created a significant economic burden, and …
The CRISPR–Cas toolbox for analytical and diagnostic assay development
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
Glycerol additive boosts 100-fold sensitivity enhancement for one-pot RPA-CRISPR/Cas12a assay
M Lin, H Yue, T Tian, E Xiong, D Zhu, Y Jiang… - Analytical …, 2022 - ACS Publications
CRISPR/Cas12, a highly efficient and specific nucleic acid recognition system, has been
broadly employed to detect amplified DNA products. However, most reported methods adopt …
broadly employed to detect amplified DNA products. However, most reported methods adopt …
CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
Enzyme kinetics and detector sensitivity determine limits of detection of amplification-free CRISPR-Cas12 and CRISPR-Cas13 diagnostics
DA Huyke, A Ramachandran, VI Bashkirov… - Analytical …, 2022 - ACS Publications
Interest in CRISPR-Cas12 and CRISPR-Cas13 detection continues to increase as these
detection schemes enable the specific recognition of nucleic acids. The fundamental …
detection schemes enable the specific recognition of nucleic acids. The fundamental …
Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2
Type VI CRISPR-Cas systems have been repurposed for various applications such as gene
knockdown, viral interference, and diagnostics. However, the identification and …
knockdown, viral interference, and diagnostics. However, the identification and …
Signal amplification and output of CRISPR/Cas-based biosensing systems: A review
SY Wang, YC Du, DX Wang, JY Ma, AN Tang… - Analytica Chimica …, 2021 - Elsevier
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-
associated) proteins are powerful gene-editing tools because of their ability to accurately …
associated) proteins are powerful gene-editing tools because of their ability to accurately …
Applications and challenges of CRISPR-Cas gene-editing to disease treatment in clinics
W Liu, L Li, J Jiang, M Wu, P Lin - Precision clinical medicine, 2021 - academic.oup.com
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated systems
(Cas) are efficient tools for targeting specific genes for laboratory research, agricultural …
(Cas) are efficient tools for targeting specific genes for laboratory research, agricultural …
Multiplexed detection of foodborne pathogens using one-pot CRISPR/Cas12a combined with recombinase aided amplification on a finger-actuated microfluidic …
G Xing, Y Shang, X Wang, H Lin, S Chen, Q Pu… - Biosensors and …, 2023 - Elsevier
Foodborne pathogens have raised significant concerns in human public health. Rapid, high-
sensitive, low-cost, and easy-to-use testing methods for food safety are needed. In this study …
sensitive, low-cost, and easy-to-use testing methods for food safety are needed. In this study …
Programmable RNA detection with CRISPR-Cas12a
SR Rananaware, EK Vesco, GM Shoemaker… - Nature …, 2023 - nature.com
Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA
substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that …
substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that …